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AB314093

Anti-FOXQ1 antibody [BLR230K] - BSA free

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Rabbit Recombinant Monoclonal FOXQ1 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human FOXQ1 aa 350 to C-terminus.

View Alternative Names

HFH1, FOXQ1, Forkhead box protein Q1, HNF-3/forkhead-like protein 1, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH-1

6 Images
Immunocytochemistry - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • ICC

Supplier Data

Immunocytochemistry - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Detection of human FOXQ1 by immunocytochemistry Sample : FFPE section of NCI-H520 cells. Antibody : Rabbit anti-FOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230). Secondary : HRP-conjugated goat antirabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Detection of human FOXQ1 by immunohistochemistry. Sample : FFPE section of human colon carcinoma. Antibody : Rabbit anti-FOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230). Secondary : HRP-conjugated goat anti-rabbit IgG.

Flow Cytometry - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Detection of human FOXQ1 (shaded) in HT-29 cells by flow cytometry. Antibody : Rabbit anti-FOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Immunoprecipitation - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • IP

Supplier Data

Immunoprecipitation - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Detection of human FOXQ1 by western blot of immunoprecipitates. Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H520 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiFOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230) used for IP at 6 µl/mg lysate. FOXQ1 was also immunoprecipitated by a second antibody against a different epitope of FOXQ1 (BL-2269A-2C5). For blotting immunoprecipitated FOXQ1, A700-230 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FOXQ1 antibody [BLR230K] - BSA free (ab314093) at 1/1000 dilution

All lanes:

NCI-H520 cells at 1 mg

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Detection of mouse FOXQ1 by immunohistochemistry. Sample : FFPE section of mouse bladder. Antibody : Rabbit anti-FOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230). Secondary : HRP-conjugated goat antirabbit IgG.

Western blot - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)
  • WB

Supplier Data

Western blot - Anti-FOXQ1 antibody [BLR230K] - BSA free (AB314093)

Samples : Whole cell lysate (50 µg) from Hep-G2, HEK293T, NCIH520, MUTZ-3, and HT-29 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-FOXQ1 recombinant monoclonal antibody [BLR230K] (A700-230) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-FOXQ1 antibody [BLR230K] - BSA free (ab314093) at 1/1000 dilution

Lane 1:

Hep-G2 Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

NCI-H520 Whole cell lysate at 50 µg

Lane 4:

MUTZ-3 Whole cell lysate at 50 µg

Lane 5:

HT-29 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR230K

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IP, IHC-P, Flow Cyt, ICC

applications

Immunogen

Synthetic Peptide within Human FOXQ1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9C009

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Assay Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXQ1 also known as Forkhead box Q1 is a transcription factor that regulates gene expression. Its molecular weight is approximately 44 kDa. FOXQ1 expression occurs in various tissues including the lungs intestines and certain parts of the brain. This protein belongs to the Forkhead box (FOX) family which plays key roles in numerous cellular processes. These transcription factors share a similar DNA-binding domain enabling binding to specific sequences and control of target gene expression.
Biological function summary

FOXQ1 influences cell proliferation differentiation and survival. It does not form part of a larger complex though it interacts with other proteins for its function. In particular FOXQ1 can modulate epithelial-mesenchymal transition (EMT) an essential process in development and cancer metastasis. Through its role in EMT FOXQ1 impacts cellular plasticity and tumor progression thereby influencing how cells respond to growth signals.

Pathways

Connections involving FOXQ1 include the TGF-beta signaling and Wnt pathways. These pathways are critical in controlling cellular growth and differentiation functions. Within these pathways FOXQ1 interacts with proteins such as SMAD and beta-catenin which further transmit signals to the nucleus altering gene expression patterns. Such interactions position FOXQ1 as a pivotal component in facilitating cellular responses to environmental stimuli.

FOXQ1 has implications in cancer and fibrotic diseases. In cancers such as colorectal and breast cancer abnormal FOXQ1 expression can lead to more aggressive tumor phenotypes and poor prognosis. FOXQ1 associates with proteins like E-cadherin and Vimentin during cancer progression influencing tumor invasiveness and metastasis. In fibrosis FOXQ1 dysregulation affects tissue repair by modifying fibroblast activity and matrix deposition showcasing its role in pathological tissue remodeling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in hair follicle differentiation.
See full target information FOXQ1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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