Anti-FPR2 antibody [HL2664] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

ab325854 detects FPRL1 protein by immunofluorescent analysis. Sample : HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : FPRL1 stained by ab325854 diluted at 1 : 100. Blue : Fluoroshield with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

ab325854 detects FPRL1 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded mouse spleen. FPRL1 stained by ab325854 diluted at 1 : 200 and competitor's antibody diluted at 1 : 200. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

ab325854 detects FPRL1 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded mouse spleen. FPRL1 stained by ab325854 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

Unboiled U937 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325854 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FPR2 antibody [HL2664] - BSA and Azide free (ab325854) at 1/1000 dilution

Lane 1:

Unboiled U937 whole cell extracts at 30 µg

Lane 2:

Unboiled U937 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

ab325854 detects FPRL1 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : FPRL1 stained by ab325854 diluted at 1 : 500 and competitor's antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

ab325854 detects FPRL1 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : FPRL1 stained by ab325854 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-FPR2 antibody [HL2664] - BSA and Azide free (AB325854)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325854 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FPR2 antibody [HL2664] - BSA and Azide free (ab325854) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged FPR2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged FPR2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false