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AB249728

Anti-FPR3 antibody [EPR11865] - BSA and Azide free

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Rabbit Recombinant Monoclonal FPR3 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples.

View Alternative Names

FPRH1, FPRL2, FPR3, N-formyl peptide receptor 3, FMLP-related receptor II, Formyl peptide receptor-like 2, FMLP-R-II

2 Images
Flow Cytometry - Anti-FPR3 antibody [EPR11865] - BSA and Azide free (AB249728)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FPR3 antibody [EPR11865] - BSA and Azide free (AB249728)

This data was developed using ab172908, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of NCI-H460 cells labeling FPR3 with ab172908 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-FPR3 antibody [EPR11865] - BSA and Azide free (AB249728)
  • WB

Supplier Data

Western blot - Anti-FPR3 antibody [EPR11865] - BSA and Azide free (AB249728)

This data was developed using ab172908, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FPR3 antibody [EPR11865] (<a href='/en-us/products/primary-antibodies/fpr3-antibody-epr11865-ab172908'>ab172908</a>) at 1/1000 dilution

Lane 1:

Human thymus at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

NCI-H460 cell lysate at 10 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11865

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249728 is the carrier-free version of ab172908.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Formyl peptide receptor 3 (FPR3) also known as FMLP-R and FPRL2 is a G protein-coupled receptor with a molecular mass of approximately 39 kDa. FPR3 is positioned on the membrane of various immune cells including monocytes and neutrophils. This receptor plays an important role in detecting and responding to chemoattractant molecules. FPR3 can capture and transmit signals through conformational changes facilitating chemotaxis and other immune responses.
Biological function summary

FPR3 influences immune cell behavior and regulates the host's defense mechanisms. It participates in the chemotactic response directing immune cells to sites of infection or inflammation. While it is not part of a larger receptor complex FPR3 functions synergistically with other formyl peptide receptors like FPR1 and FPR2 to fine-tune immune responses. These coordinated activities help in controlling inflammation and microbial defense.

Pathways

Researchers have identified FPR3 as a significant component of the chemotaxis pathway in immune signaling. It interacts with key intracellular signaling molecules leading to the modulation of the MAPK signaling pathway. This coordination enhances immune cell mobility and function. FPR3 shares functional characteristics with related proteins such as FPR1 which also participates in the regulation of immune responses necessary for host defense.

FPR3 shows involvement in inflammatory conditions and certain types of cancer. Aberrations in FPR3 signaling may contribute to chronic inflammation impacting diseases like rheumatoid arthritis. Its dysregulation can affect cancer progression particularly in scenarios involving tumor-associated macrophages. The interactions between FPR3 and related immune proteins like FPR1 can influence the severity and prognosis of these conditions by modifying the immune environment in pathological tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Acts as a receptor for humanin (PubMed : 15465011).
See full target information FPR3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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