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AB63022

Anti-FPRL1/RFP antibody

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(6 Publications)

Rabbit Polyclonal FPRL1/RFP antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FPR2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FPRH1, FPRL1, LXA4R, FPR2, N-formyl peptide receptor 2, FMLP-related receptor I, Formyl peptide receptor-like 1, HM63, Lipoxin A4 receptor, RFP, FMLP-R-I, LXA4 receptor

3 Images
Flow Cytometry - Anti-FPRL1/RFP antibody (AB63022)
  • Flow Cyt

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Flow Cytometry - Anti-FPRL1/RFP antibody (AB63022)

Flow cytometric analysis of HL60 cells using ab63022 at 1 : 100 (red) compared to a non-specific negative control antibody (black).

Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody (AB63022)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody (AB63022)

Immunofluorescent analysis of HL60 cells using ab63022 (green) at 1 : 500. Nucleus have bee labeled with PI (red).

Western blot - Anti-FPRL1/RFP antibody (AB63022)
  • WB

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Western blot - Anti-FPRL1/RFP antibody (AB63022)

All lanes:

Western blot - Anti-FPRL1/RFP antibody (ab63022) at 1/5000 dilution

All lanes:

Extracts from HL60 cells.

Predicted band size: 39 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human FPR2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25090

Specificity

This antibody recognizes human FPRL-1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FPRL1 known also as FPR2 or formyl peptide receptor-like 1 is a G-protein-coupled receptor with an approximate mass of 39 kDa. This receptor primarily resides on the surface of immune cells such as neutrophils monocytes and dendritic cells. FPRL1 plays an important role in transmitting chemotactic signals that guide immune cells towards sites of inflammation or infection. It binds ligands that include formylated peptides and lipid mediators initiating a cascade of intracellular signals that mobilize immune responses.
Biological function summary

FPRL1 enables the immune system to detect and respond to pathogenic threats effectively. As part of the immune surveillance mechanism FPRL1 aids in the migration and function of immune cells by activating intracellular second messenger systems including calcium mobilization and MAPK pathways. Though not part of a larger protein complex it interacts closely with other signaling molecules to amplify immune responses. The receptor also shows diverse ligand recognition possibly allowing it to participate in different immune processes.

Pathways

FPRL1 holds a position in both inflammatory and resolution pathways. Notably it participates in the chemokine signaling pathway working alongside other G-protein-coupled receptors to regulate leukocyte chemotaxis. FPRL1 further involves itself in the resolution of inflammation through interactions with specialized pro-resolving mediators like lipoxins. An example of this interaction occurs with the protein ALX/FPR2 with which FPRL1 shares functional similarities contributing to both swift inflammatory responses and their resolution.

FPRL1 implicates itself in chronic inflammatory diseases and cancer. In chronic inflammatory conditions FPRL1 overstimulation may contribute to prolonged inflammation adversely affecting tissue function. Furthermore aberrant signaling through FPRL1 potentially influences tumor progression in various cancers. FPRL1 can interact with PD-L1 within the tumor microenvironment promoting immune evasion in cancer cells. Understanding these interactions offers potential therapeutic targets to modulate immune responses and treat associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophil chemotactic factors (PubMed : 1374236). Binding of FMLP to the receptor causes activation of neutrophils (PubMed : 1374236). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 1374236). The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of pro-inflammatory signals such as LTB4 (leukotriene B4) (PubMed : 9547339). Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation (By similarity). Acts as a receptor for humanin (PubMed : 15465011).
See full target information FPR2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of assisted reproduction and genetics 39:239-250 PubMed35018584

2022

Suppression of FPR2 expression inhibits inflammation in preeclampsia by improving the biological functions of trophoblast via NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxian Li,Anna Li,Liping Zhai,Yaqiong Sun,Ling Yu,Zhenya Fang,Lin Zhang,Yanjie Peng,Meihua Zhang,Xietong Wang

Journal of thrombosis and haemostasis : JTH 15:802-813 PubMed28079976

2017

Maresin 1 induces a novel pro-resolving phenotype in human platelets.

Applications

Unspecified application

Species

Unspecified reactive species

K L Lannan,S L Spinelli,N Blumberg,R P Phipps

Cell proliferation 50: PubMed27910163

2016

Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yu,Shujie Zhang,Jiajing Lu,Ying Li,Xuemei Yi,Li Tang,Lina Su,Yangfeng Ding

The American journal of pathology 185:3189-201 PubMed26468975

2015

Resolvin D1 Reduces Emphysema and Chronic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Hsi-Min Hsiao,Thomas H Thatcher,Romain A Colas,Charles N Serhan,Richard P Phipps,Patricia J Sime

Immunology 142:474-83 PubMed24617769

2014

The HIV-1 gp41 ectodomain is cleaved by matriptase to produce a chemotactic peptide that acts through FPR2.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew P Wood,Amy L Cole,Colleen R Eade,Li-Mei Chen,Karl X Chai,Alexander M Cole

Fertility and sterility 99:1965-73.e2 PubMed23472950

2013

Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4).

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Gori,Yoima Rodriguez,Chiara Pellegrini,Chahin Achtari,Daniela Hornung,Eric Chardonnens,Dorothea Wunder,Maryse Fiche,Geraldine O Canny
View all publications
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