AB26316

Anti-FPRL1/RFP antibody [GM1D6]

Name: Anti-FPRL1/RFP antibody [GM1D6] (ab26316) | Abcam
Brand: Abcam Limited
SKU: AB26316
Price: 500 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(10 Publications)

Mouse Monoclonal FPRL1/RFP antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

FPRH1, FPRL1, LXA4R, FPR2, N-formyl peptide receptor 2, FMLP-related receptor I, Formyl peptide receptor-like 1, HM63, Lipoxin A4 receptor, RFP, FMLP-R-I, LXA4 receptor

2 Images

Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

Flow Cyt

Unknown

Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1/RFP (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

ab26316 staining of FPRL1/RFP in CHO cells using 1μg/10⁶ cells. CHO cells were transiently transfected with an expession vector encoding FPRL1/RFP and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GM1D6

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Ab26316 recognises FPRL1/RFP transiently expressed on the cell surface of transfected BOSC cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/cells for 6 µg/cells", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FPRL1 known also as FPR2 or formyl peptide receptor-like 1 is a G-protein-coupled receptor with an approximate mass of 39 kDa. This receptor primarily resides on the surface of immune cells such as neutrophils monocytes and dendritic cells. FPRL1 plays an important role in transmitting chemotactic signals that guide immune cells towards sites of inflammation or infection. It binds ligands that include formylated peptides and lipid mediators initiating a cascade of intracellular signals that mobilize immune responses.

Biological function summary

FPRL1 enables the immune system to detect and respond to pathogenic threats effectively. As part of the immune surveillance mechanism FPRL1 aids in the migration and function of immune cells by activating intracellular second messenger systems including calcium mobilization and MAPK pathways. Though not part of a larger protein complex it interacts closely with other signaling molecules to amplify immune responses. The receptor also shows diverse ligand recognition possibly allowing it to participate in different immune processes.

Pathways

FPRL1 holds a position in both inflammatory and resolution pathways. Notably it participates in the chemokine signaling pathway working alongside other G-protein-coupled receptors to regulate leukocyte chemotaxis. FPRL1 further involves itself in the resolution of inflammation through interactions with specialized pro-resolving mediators like lipoxins. An example of this interaction occurs with the protein ALX/FPR2 with which FPRL1 shares functional similarities contributing to both swift inflammatory responses and their resolution.

FPRL1 implicates itself in chronic inflammatory diseases and cancer. In chronic inflammatory conditions FPRL1 overstimulation may contribute to prolonged inflammation adversely affecting tissue function. Furthermore aberrant signaling through FPRL1 potentially influences tumor progression in various cancers. FPRL1 can interact with PD-L1 within the tumor microenvironment promoting immune evasion in cancer cells. Understanding these interactions offers potential therapeutic targets to modulate immune responses and treat associated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophil chemotactic factors (PubMed : 1374236). Binding of FMLP to the receptor causes activation of neutrophils (PubMed : 1374236). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 1374236). The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of pro-inflammatory signals such as LTB4 (leukotriene B4) (PubMed : 9547339). Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation (By similarity). Acts as a receptor for humanin (PubMed : 15465011).

See full target information FPR2

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22920 PubMed37078546

2023

Glutamatergic melanocortin-4 receptor neurons regulate body weight.

Applications

Unspecified application

Species

Unspecified reactive species

Haodong Liu,Xiaojing Li,Penghui Li,Rihan Hai,Jiacheng Li,Qi Fan,Xing Wang,Yujie Chen,Xiaojuan Cao,Xiaoyu Zhang,Ruifeng Gao,Kun Wang,Chenguang Du

FASEB bioAdvances 2:613-623 PubMed33089077

2020

The G-protein-coupled chemoattractant receptor Fpr2 exacerbates neuroglial dysfunction and angiogenesis in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yu,Shengding Xue,Keqiang Chen,Yingying Le,Rongrong Zhu,Shiyi Wang,Shuang Liu,Xinliang Cheng,Huaijin Guan,Ji Ming Wang,Hui Chen

Nature communications 11:4243 PubMed32843625

2020

Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Jäger,Supriya Murthy,Caroline Schmidt,Magdalena Hahn,Sarah Strobel,Anna Peters,Claudia Stäubert,Pelin Sungur,Tom Venus,Mandy Geisler,Veselina Radusheva,Stefanie Raps,Kathrin Rothe,Roger Scholz,Sebastian Jung,Sylke Wagner,Matthias Pierer,Olga Seifert,Wenhan Chang,Irina Estrela-Lopis,Nora Raulien,Knut Krohn,Norbert Sträter,Stephanie Hoeppener,Torsten Schöneberg,Manuela Rossol,Ulf Wagner

The American journal of pathology 189:2258-2268 PubMed31437425

2019

Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie G Dakin,Romain A Colas,Kim Wheway,Bridget Watkins,Louise Appleton,Jonathan Rees,Stephen Gwilym,Christopher Little,Jesmond Dalli,Andrew J Carr

FASEB journal : official publication of the Federa 33:8043-8054 PubMed30916999

2019

15-Epi-LXA and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie G Dakin,Romain A Colas,Julia Newton,Stephen Gwilym,Natasha Jones,Hamish A B Reid,Simon Wood,Louise Appleton,Kim Wheway,Bridget Watkins,Jesmond Dalli,Andrew J Carr

Scientific reports 7:7743 PubMed28798364

2017

Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses.

Applications

Species

Horse

Nicolas Herteman,Amandine Vargas,Jean-Pierre Lavoie

Journal of immunology (Baltimore, Md. : 1950) 193:4980-7 PubMed25320283

2014

Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1.

Applications

Unspecified application

Species

Unspecified reactive species

Hsi-Min Hsiao,Thomas H Thatcher,Elizabeth P Levy,Robert A Fulton,Kristina M Owens,Richard P Phipps,Patricia J Sime

PloS one 7:e48978 PubMed23155437

2012

Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease.

Applications

IHC-Fr

Species

Horse

Stephanie Georgina Dakin,Jayesh Dudhia,Natalie Jayne Werling,Dirk Werling,Dilkush Robert Ephrem Abayasekara,Roger Kenneth Whealands Smith

PloS one 7:e32333 PubMed22384219

2012

Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair.

Applications

IHC-Fr

Species

Horse

Stephanie Georgina Dakin,Dirk Werling,Andrew Hibbert,Dilkush Robert Ephrem Abayasekara,Natalie Jayne Young,Roger Kenneth Whealands Smith,Jayesh Dudhia

Journal of leukocyte biology 83:245-53 PubMed17984291

2007

Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Björkman,Jennie Karlsson,Anna Karlsson,Marie-Josèphe Rabiet,Francois Boulay,Huamei Fu,Johan Bylund,Claes Dahlgren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FPRL1/RFP antibody [GM1D6]

Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (AB26316)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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