Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FPRL1/RFP + FPR3 with ab317815 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human cerebrum. The section was incubated with ab317815 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a FPR2 expression vector containing a Myc-His tag. (B) HEK-293T transfected with FPR3 expression vector containing a Myc-His tag. (C) HEK-293T transfected with a FPR1 expression vector containing a Myc-His tag. (D) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling FPRL1/RFP + FPR3 with ab317815 at 1/2000 (0.246 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

This antibody recognizes both FPR2 and FPR3. Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a FPR2 expression vector containing a Myc-His tag and (B) HEK-293T transfected with a FPR3 expression vector containing a Myc-His tag negative staining on (C) HEK-293T transfected with a FPR1 expression vector containing a Myc-His tag and (D) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab317815 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling FPRL1/RFP + FPR3 with ab317815 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neutrophils of human lung. The section was incubated with ab317815 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling FPRL1/RFP + FPR3 with ab317815 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neutrophils of human spleen (PMID : 9151906). The section was incubated with ab317815 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FPRL1/RFP + FPR3 with ab317815 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neutrophils of human cardiac muscle. The section was incubated with ab317815 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP + FPR3 antibody [EPR28515-94] (AB317815)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling FPRL1/RFP + FPR3 with ab317815 at 1/100 (4.91 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in CD14 positive cells of human PBMC (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Anti-human CD14 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.