Anti-FRAS1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-FRAS1 antibody (AB240583)

FRAS1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. Western blot was performed from the immunoprecipitate using ab240583 at 1/100 dilution.

Lane 1 : ab240583 used for IP at 20 μl per reaction.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FRAS1 antibody (ab240583)

Predicted band size: 443 kDa

false

• WB

Supplier Data

Western blot - Anti-FRAS1 antibody (AB240583)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FRAS1 antibody (ab240583) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 443 kDa

true

Exposure time: 3min