Rabbit Polyclonal FRAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FRAT1 aa 200 to C-terminus.
Images
Publications
- Thoracic cancer 11:295-3042019LncRNA SNHG1 influences cell proliferation, migration, invasion, and apoptosis of non-small cell lung cancer cells via the miR-361-3p/FRAT1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomei Li,Hong ZhengPubMed 31788970
- Molecular medicine reports 14:3551-82016Frequently rearranged in advanced T‑cell lymphomas‑1 demonstrates oncogenic properties in prostate cancer.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human FRAT1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92837
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. May play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis.
Alternative names
Proto-oncogene FRAT1, Frequently rearranged in advanced T-cell lymphomas 1, FRAT-1, FRAT1
Recommended products
Rabbit Polyclonal FRAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FRAT1 aa 200 to C-terminus.
Alternative names
Proto-oncogene FRAT1, Frequently rearranged in advanced T-cell lymphomas 1, FRAT-1, FRAT1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human FRAT1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92837
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
FRAT1 also known as Frequently Rearranged in Advanced T-cell lymphomas 1 is a regulatory protein involved in the Wnt signaling pathway. It has a molecular mass of approximately 29 kDa. The protein expresses mainly in tissues such as the brain and lymphoid organs. FRAT1 functions as a positive regulator of the Wnt pathway by inhibiting GSK-3 beta leading to the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus. This role is significant in mediating gene transcription related to cell proliferation and differentiation.
- Biological function summary
FRAT1 contributes to cellular and developmental processes. It forms a functional complex with other proteins in the Wnt signaling pathway including Dishevelled proteins and Axin. The interaction within this complex aids in the modulation of β-catenin levels which is a critical step in the transmission of Wnt signals from the cell surface to the nucleus. Its regulation is necessary for maintaining normal developmental processes and cellular homeostasis.
- Pathways
FRAT1 plays an important role in the Wnt/β-catenin signaling and the cell proliferation pathway. It interacts with proteins such as GSK-3 beta and β-catenin directly affecting the progression of Wnt signals. Through this signaling pathway FRAT1 influences cell fate decisions contributing to tissue development and regeneration. The regulation of these pathways by FRAT1 is essential for controlling cellular proliferation and differentiation in various tissues.
- Associated diseases and disorders
FRAT1 has been implicated in cancer specifically in the development of certain lymphomas and gliomas. Its overexpression or mutation can lead to dysregulated Wnt/β-catenin signaling which contributes to tumorigenesis and the progression of these diseases. Additionally alterations in FRAT1 activity may affect proteins like β-catenin which can further influence cancer-associated signaling networks. Understanding the role of FRAT1 in these pathological conditions may offer insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-FRAT1 antibody - C-terminal (ab137391)
12% SDS PAGE
All lanes: Western blot - Anti-FRAT1 antibody - C-terminal (ab137391) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRAT1 antibody - C-terminal (ab137391)
Immunohistochemical analysis of paraffin-embedded Human SG xenograft tissue, labeling FRAT1 with ab137391 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody - C-terminal (ab137391)
Immunofluorescence analysis of methanol-fixed A431 cells, labelling FRAT1 with ab137391 at 1/200 dilution. Lower image is costained with Hoechst 33342.
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