AB108405

Anti-FRAT1 antibody [EPR3900]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(9 Publications)

Rabbit Recombinant Monoclonal FRAT1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Proto-oncogene FRAT1, Frequently rearranged in advanced T-cell lymphomas 1, FRAT-1, FRAT1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (AB108405)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (AB108405)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling FRAT1 with purified ab108405 at 1/250 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Unknown

Western blot - Anti-FRAT1 antibody [EPR3900] (AB108405)

All lanes:

Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Predicted band size: 29 kDa

false

CiteAb

Western blot - Anti-FRAT1 antibody [EPR3900] (AB108405)

FRAT1 western blot using anti-FRAT1 antibody [EPR3900] ab108405. Publication image and figure legend from Werner, T. V., Hart, M., et al., 2017, Aging (Albany NY), PubMed 28340489.

ab108405 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108405 please see the product overview.

Western blot for SMAD4, FRAT1 and BCL2 protein expression after deregulation of miR-34a-3p in Ben-Men-1 cells(A, B, C) Representative Western blot images for SMAD4, FRAT1 and BCL2 protein expression 48 h after transfection of Ben-Men-1 cells with control (scrambled), miR-34a-3p (mimic) or anti-miR-34a-3p (inhibitor) using β-actin as loading control. (D, E, F) Quantification of SMAD4, FRAT1 and BCL2 protein expression relative to β-actin protein expression determined by Western blot. Values are means ± SD of three independent experiments (*, p < 0.05; **, p < 0.01).

false

Carrier free

Anti-FRAT1 antibody [EPR3900] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3900

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRAT1 also known as Frequently Rearranged in Advanced T-cell lymphomas 1 is a regulatory protein involved in the Wnt signaling pathway. It has a molecular mass of approximately 29 kDa. The protein expresses mainly in tissues such as the brain and lymphoid organs. FRAT1 functions as a positive regulator of the Wnt pathway by inhibiting GSK-3 beta leading to the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus. This role is significant in mediating gene transcription related to cell proliferation and differentiation.

Biological function summary

FRAT1 contributes to cellular and developmental processes. It forms a functional complex with other proteins in the Wnt signaling pathway including Dishevelled proteins and Axin. The interaction within this complex aids in the modulation of β-catenin levels which is a critical step in the transmission of Wnt signals from the cell surface to the nucleus. Its regulation is necessary for maintaining normal developmental processes and cellular homeostasis.

Pathways

FRAT1 plays an important role in the Wnt/β-catenin signaling and the cell proliferation pathway. It interacts with proteins such as GSK-3 beta and β-catenin directly affecting the progression of Wnt signals. Through this signaling pathway FRAT1 influences cell fate decisions contributing to tissue development and regeneration. The regulation of these pathways by FRAT1 is essential for controlling cellular proliferation and differentiation in various tissues.

FRAT1 has been implicated in cancer specifically in the development of certain lymphomas and gliomas. Its overexpression or mutation can lead to dysregulated Wnt/β-catenin signaling which contributes to tumorigenesis and the progression of these diseases. Additionally alterations in FRAT1 activity may affect proteins like β-catenin which can further influence cancer-associated signaling networks. Understanding the role of FRAT1 in these pathological conditions may offer insights into potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. May play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis.

See full target information Proto-oncogene FRAT1

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38891118

2024

Ileal Crohn's Disease Exhibits Reduced Activity of Phospholipase C-β3-Dependent Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoaki Ando,Ikuo Takazawa,Zachary T Spencer,Ryoji Ito,Yoshiaki Tomimori,Zbigniew Mikulski,Kenji Matsumoto,Tohru Ishitani,Lee A Denson,Yu Kawakami,Yuko Kawakami,Jiro Kitaura,Yashi Ahmed,Toshiaki Kawakami

Aging 13:18527-18544 PubMed34319909

2021

LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Cai,Yiheng Dai,Peng Gao,Guanyu Ren,Dingcai Cheng,Bo Wang,Yi Wang,Jiang Yu,Yiheng Du,Xizhi Wang,Boxin Xue

Oncology letters 20:248-256 PubMed32565951

2020

STAT1 is a modulator of the expression of frequently rearranged in advanced T-cell lymphomas 1 expression in U251 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Guo,Shule Wang,Yining Hao,Yeqing Ren,Yongqiang Wu,Jianping Zhang,Dong Liu

CPT: pharmacometrics & systems pharmacology 7:16-25 PubMed28925077

2017

Network-Wide Screen Identifies Variation of Novel Precise On-Module Targets Using Conformational Modudaoism.

Applications

Species

Unspecified reactive species

Bing Li,Jun Liu,Yanan Yu,Pengqian Wang,Yingying Zhang,Xumin Ni,Qiong Liu,Xiaoxu Zhang,Zhong Wang,Yongyan Wang

Aging 9:932-954 PubMed28340489

2017

MiR-34a-3p alters proliferation and apoptosis of meningioma cells and is directly targeting SMAD4, FRAT1 and BCL2.

Applications

Species

Unspecified reactive species

Tamara V Werner,Martin Hart,Ruth Nickels,Yoo-Jin Kim,Michael D Menger,Rainer M Bohle,Andreas Keller,Nicole Ludwig,Eckart Meese

Oncology letters 12:4761-4766 PubMed28101222

2016

FRAT1 expression regulates proliferation in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kongxi Zhu,Jianqiang Guo,Hongjuan Wang,Weihua Yu

Molecular medicine reports 14:3551-8 PubMed27599661

2016

Frequently rearranged in advanced T‑cell lymphomas‑1 demonstrates oncogenic properties in prostate cancer.

Applications

Species

Unspecified reactive species

Wei Zhang,Hua Xiong,Yanmei Zou,Sanpeng Xu,Lanping Quan,Xianglin Yuan,Ningzhi Xu,Yihua Wang

Biomedical reports 4:223-226 PubMed26893843

2016

Silencing of FRAT1 by siRNA inhibits the proliferation of SGC7901 human gastric adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qinggong Yu,L U Shang,Hongbo Yu,Zirong Yang,Dekui Xu

Journal of cell science 127:3116-30 PubMed24829151

2014

The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of β-catenin through mechanisms involving FRAT1 and PAK4.

Applications

Unspecified application

Species

Human

Runsen Jin,Wensheng Liu,Sharleen Menezes,Fei Yue,Minhua Zheng,Zaklina Kovacevic,Des R Richardson

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FRAT1 antibody [EPR3900]

Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (AB108405)

Western blot - Anti-FRAT1 antibody [EPR3900] (AB108405)

Western blot - Anti-FRAT1 antibody [EPR3900] (AB108405)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise