Anti-Frataxin antibody [18A5DB1] is a mouse monoclonal antibody that is used to detect Frataxin in Flow cytometry, ICC/IF, IHC-P, Western blot. Suitable for Human samples.
- Cited in over 35 publications
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Flow Cyt | WB | ICC/IF | IHC-P | |
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Human | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info 1 µg/mL | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 3-5 µg/mL | Notes (A chemiluminescent detection system is highly recommended) |
Species | Dilution info | Notes |
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Species Human | Dilution info 0.5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
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Frataxin mature form. Functions as an activator of persulfide transfer to the scaffoding protein ISCU as component of the core iron-sulfur cluster (ISC) assembly complex and participates to the [2Fe-2S] cluster assembly (PubMed:12785837, PubMed:24971490). Accelerates sulfur transfer from NFS1 persulfide intermediate to ISCU and to small thiols such as L-cysteine and glutathione leading to persulfuration of these thiols and ultimately sulfide release (PubMed:24971490). Binds ferrous ion and is released from FXN upon the addition of both L-cysteine and reduced FDX2 during [2Fe-2S] cluster assembly (PubMed:29576242). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity (PubMed:15641778). May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems (PubMed:11823441, PubMed:12755598). May function as an iron chaperone protein that protects the aconitase [4Fe-4S]2+ cluster from disassembly and promotes enzyme reactivation (PubMed:15247478). May play a role as a high affinity iron binding partner for FECH that is capable of both delivering iron to ferrochelatase and mediating the terminal step in mitochondrial heme biosynthesis (PubMed:15123683, PubMed:16239244). Extramitochondrial frataxin. Modulates the RNA-binding activity of ACO1 (PubMed:20053667). May be involved in the cytoplasmic iron-sulfur protein biogenesis (PubMed:16091420). May contribute to oxidative stress resistance and overall cell survival (PubMed:16608849).
FRDA, X25, FXN, Friedreich ataxia protein, Fxn
Anti-Frataxin antibody [18A5DB1] is a mouse monoclonal antibody that is used to detect Frataxin in Flow cytometry, ICC/IF, IHC-P, Western blot. Suitable for Human samples.
- Cited in over 35 publications
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
ab110328 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Although this antibody does work in Western blot, we have found that Anti-Frataxin antibody [17A11] ab113691 typically gives a more robust signal. Therefore, if you plan on using this antibody in WB only, we recommend Anti-Frataxin antibody [17A11] ab113691 as an alternative.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-Frataxin antibody [18A5DB1] (ab110328) at 5 µg/mL
All lanes: Recombinant Human Frataxin at 0.0005 µg
Predicted band size: 23 kDa
Flow cytometric analysis using ab110328 at 1µg/ml staining Frataxin in HL60 cells (blue). Isotype control antibody (red).
Immunofluorescent analysis of paraformaldehyde-fixed, 0.2% NP-40 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Frataxin with ab110328 at 1/500 dilution, followed by Goat anti-MouseAlexaFluor® 594 conjugated secondary antibody at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
IHC image of Frataxin staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110328, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry analysis using ab110328 at 0.5μg/ml staining Frataxin in normal Human fibroblasts (MRC5) (fixed and permeabilized) followed by an AlexaFluor® 594-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml). .
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