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AB181083

Anti-FRG1 antibody [EPR13098]

5

(2 Reviews)

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(1 Publication)

Rabbit Recombinant Monoclonal FRG1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Protein FRG1, FSHD region gene 1 protein, FRG1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] (AB181083)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] (AB181083)

Immunofluorescent analysis of HeLa cells labeling FRG1 with ab181083 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 555-conjugated goat anti-rabbit lgG (red - left panel). DAPI was used to stain the cell nuclear (blue - right panel).

Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (AB181083)
  • IP

Supplier Data

Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (AB181083)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling FRG1 with ab181083 at 1/30 dilution. Right lane is the negative control.

All lanes:

Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (ab181083)

Predicted band size: 29 kDa

false

Western blot - Anti-FRG1 antibody [EPR13098] (AB181083)
  • WB

Supplier Data

Western blot - Anti-FRG1 antibody [EPR13098] (AB181083)

All lanes:

Western blot - Anti-FRG1 antibody [EPR13098] (ab181083) at 1/10000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

Jurkat whole cell lysate at 20 µg

Lane 3:

293 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

false

  • Carrier free

    Anti-FRG1 antibody [EPR13098] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13098

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRG1 also known as FSHD Region Gene 1 is a protein with a molecular mass of approximately 30 kDa. It is expressed in a range of human tissues with notable expression in skeletal muscle and embryonic tissues. FRG1 plays a role in cellular activities related to the maintenance and function of muscle cells. Its involvement is linked to ribonucleoprotein complex assembly and it has potential implications in cellular localization of proteins.
Biological function summary

FRG1 is involved in processes related to muscle development and maintenance. It is a part of the spliceosome complex which plays an important role in mRNA splicing. Through its participation in this complex FRG1 helps regulate the maturation of pre-mRNA by removing introns. These activities are essential for accurate gene expression in cells particularly in tissues requiring precise regulation like muscle fibers.

Pathways

Four central components relate closely to FRG1's function. FRG1 is integrated into the mRNA processing and splicing pathways which are fundamental for synthesizing functional proteins. It interacts with proteins such as SMN1 which is associated with spinal muscular atrophy highlighting its connection to the ribonucleoprotein assembly pathway. Through these pathways FRG1 contributes to ensuring proper gene expression and cellular homeostasis.

Two prominent conditions involve FRG1: Facioscapulohumeral muscular dystrophy (FSHD) and certain myopathies. FSHD is associated with the abnormal expression of FRG1 leading to progressive muscle weakness. Other studies suggest FRG1 may also have interactions with DUX4 a protein implicated in FSHD pathogenesis. Understanding FRG1's role in these diseases provides insights into their molecular mechanisms supporting the development of therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to mRNA in a sequence-independent manner. May play a role in regulation of pre-mRNA splicing or in the assembly of rRNA into ribosomal subunits. May be involved in mRNA transport. May be involved in epigenetic regulation of muscle differentiation through regulation of activity of the histone-lysine N-methyltransferase KMT5B.
See full target information FRG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:62-73 PubMed35013556

2022

The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Dragomir B Krastev,Shudong Li,Yilun Sun,Andrew J Wicks,Gwendoline Hoslett,Daniel Weekes,Luned M Badder,Eleanor G Knight,Rebecca Marlow,Mercedes Calvo Pardo,Lu Yu,Tanaji T Talele,Jiri Bartek,Jyoti S Choudhary,Yves Pommier,Stephen J Pettitt,Andrew N J Tutt,Kristijan Ramadan,Christopher J Lord
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com