Anti-FRG1 antibody [EPR13098] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] - BSA and Azide free (AB250333)

This data was developed using ab181083, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling FRG1 with ab181083 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 555-conjugated goat anti-rabbit lgG (red - left panel). DAPI was used to stain the cell nuclear (blue - right panel).

• WB

Supplier Data

Western blot - Anti-FRG1 antibody [EPR13098] - BSA and Azide free (AB250333)

This data was developed using ab181083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FRG1 antibody [EPR13098] (<a href='/en-us/products/primary-antibodies/frg1-antibody-epr13098-ab181083'>ab181083</a>) at 1/10000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

Jurkat whole cell lysate at 20 µg

Lane 3:

293 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-FRG1 antibody [EPR13098] - BSA and Azide free (AB250333)

This data was developed using ab181083, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling FRG1 with ab181083 at 1/30 dilution. Right lane is the negative control.

All lanes:

Immunoprecipitation - Anti-FRG1 antibody [EPR13098] (<a href='/en-us/products/primary-antibodies/frg1-antibody-epr13098-ab181083'>ab181083</a>)

Predicted band size: 29 kDa

false