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AB150477

Anti-Frizzled 2/FZD2 antibody

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(2 Publications)

Rabbit Polyclonal Frizzled 2/FZD2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FZD2.

View Alternative Names

Frizzled-2, Fz-2, hFz2, FzE2, FZD2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 2/FZD2 antibody (AB150477)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 2/FZD2 antibody (AB150477)

Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelling Frizzled 2/FZD2 with ab150477 antibody at a concentration of 8 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FZD2. The exact immunogen used to generate this antibody is proprietary information.

Q14332

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except FZD7 (60%), DMAP1 (40%).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "8 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled 2 (FZD2) is a member of the Frizzled receptor family which plays a role in the Wnt signaling pathway. FZD2 also known by alternate names like FzE2 and HFzd2r is a membrane-bound receptor with a mass of approximately 70 kDa. This protein is expressed in various tissues including the brain heart and lungs indicating its involvement in multiple cellular processes. The receptor contains a distinct structure comprising seven transmembrane domains which facilitates its interaction with other molecules and downstream signaling components.
Biological function summary

FZD2 acts as a critical mediator within the Wnt signaling pathway and it often functions as part of the Frizzled receptor complex. This protein binds to secreted Wnt ligands initiating intracellular signaling cascades that regulate cell fate proliferation and differentiation. Through these interactions FZD2 influences embryonic development and tissue homeostasis. The receptor's role in these fundamental processes highlights its importance in maintaining proper cellular and organismal function.

Pathways

FZD2 participates in the canonical Wnt/β-catenin and non-canonical Wnt signaling pathways. In the canonical pathway FZD2 assists in the stabilization and accumulation of β-catenin in the cytoplasm which ultimately translocates to the nucleus to regulate gene expression. In the non-canonical pathway FZD2 plays a part in the planar cell polarity and Wnt/Ca2+ pathways. Proteins like Dishevelled (DVL) and LRP5/6 are closely related to FZD2 facilitating its role in diverse signaling events important for development and cellular dynamics.

FZD2's involvement in the Wnt signaling pathway associates it with conditions like cancer and osteoporosis. Aberrant FZD2 signaling can contribute to tumorigenesis since alterations in Wnt pathways often lead to uncontrolled cell division and cancer progression. Similarly defects in Wnt-mediated bone formation can link FZD2 to osteoporosis. In these contexts FZD2 interacts with proteins such as β-catenin and Dkk1 which help mediate its effects on disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed : 25759469). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium (PubMed : 27680706, PubMed : 29748286). TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling (PubMed : 29748286).
See full target information FZD2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 238:2361-2372 PubMed37565545

2023

Mettl14-mediated m A modification regulates the abnormal differentiation of small intestinal epithelial stem cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Yue Han,Ming-Quan Song,Li Chen

Nature 538:350-355 PubMed27680706

2016

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Tao,Jie Zhang,Paul Meraner,Alessio Tovaglieri,Xiaoqian Wu,Ralf Gerhard,Xinjun Zhang,William B Stallcup,Ji Miao,Xi He,Julian G Hurdle,David T Breault,Abraham L Brass,Min Dong
View all publications
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