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AB277797

Anti-Frizzled 4 antibody [22HCLC]

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(1 Publication)

Rabbit Recombinant Multiclonal Frizzled 4 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Frizzled-4 aa 1-250.

View Alternative Names

CD344, Frizzled-4, Fz-4, hFz4, FzE4, FZD4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 4 antibody [22HCLC] (AB277797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 4 antibody [22HCLC] (AB277797)

Immunohistochemistry analysis of FZD4 showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a FZD4 Recombinant Rabbit Multiclonal Antibody (ab277797) diluted in 3% BSA-PBS at a dilution of 1/100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunocytochemistry - Anti-Frizzled 4 antibody [22HCLC] (AB277797)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Frizzled 4 antibody [22HCLC] (AB277797)

Immunofluorescence was performed on fixed and permeabilized T-47D cells for detection of FZD-4 using Anti- FZD-4 Recombinant Rabbit Polyclonal Antibody (ab277797, 2 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of FZD-4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of FZD-4. Panel e) represents control cells with no primary antibody to assess background.

Western blot - Anti-Frizzled 4 antibody [22HCLC] (AB277797)
  • WB

Supplier Data

Western blot - Anti-Frizzled 4 antibody [22HCLC] (AB277797)

All lanes:

Western blot - Anti-Frizzled 4 antibody [22HCLC] (ab277797) at 2 µg/mL

Lane 1:

HCT 116 cell extract

Lane 2:

HCT116 membrane extract

Lane 3:

T47D cell extract

Lane 4:

T47D membrane extract

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

22HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human Frizzled-4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9ULV1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled-4 also known as FZD4 is a protein important for the Wnt signaling pathway acting as a receptor for Wnt proteins. With a mass approximately 70 kDa FZD4 is a seven-transmembrane domain protein that is part of the G-protein-coupled receptor superfamily. Expression of FZD4 has been detected mostly in tissues like the retina and vasculature which suggests its role in the development and maintenance of these systems.
Biological function summary

Frizzled-4 plays a role in mediating signaling pathways that are essential for angiogenesis and retinal vascular development. It functions as a component of a larger receptor complex that includes co-receptors such as low-density lipoprotein receptor-related proteins (LRP5/6) and Disheveled proteins. This interaction is key for transmitting Wnt ligand signals to inside the cell influencing gene expression that drives cellular processes like proliferation and differentiation.

Pathways

The FZD4 protein is integral to the canonical and non-canonical Wnt signaling pathways. It actively participates in the β-catenin pathway which governs gene expression regulation by stabilizing β-catenin a process influencing various cellular activities. Moreover FZD4 is associated with the planar cell polarity pathway which is important for cell orientation processes. These pathways connect FZD4 to several other proteins like LRP5/6 and Disheveled which work in tandem to ensure proper signal transduction.

Mutations or dysregulation of Frizzled-4 are linked to familial exudative vitreoretinopathy (FEVR) a genetic disorder affecting retinal vascular development and leading to vision loss. The protein's interaction with LRP5 is significant in this context as mutations in either protein can disrupt their synergistic function resulting in the disease. Additionally aberrations in FZD4 signaling have implications in tumorigenesis particularly in cancers where Wnt signaling is pathologically activated suggesting that Frizzled-4’s role is important in maintaining tissue homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for Wnt proteins (PubMed : 30135577). Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed : 30135577). Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
See full target information Frizzled-4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oral diseases 29:1979-1990 PubMed35403775

2022

Circ_0001971 makes progress of oral squamous cell carcinoma by targeting miR-107/FZD4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Lu,Hongguo Xie
View all publications
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Product promise

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