AB325855

Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Frizzled 4 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Zebrafish, Human samples. Immunogen corresponding to Synthetic Peptide within Human Frizzled-4.

View Alternative Names

CD344, Frizzled-4, Fz-4, hFz4, FzE4, FZD4

5 Images

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Boiled and unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/1000 dilution

Lane 1:

Boiled HepG2 whole cell extracts at 30 µg

Lane 2:

Boiled Jurkat whole cell extracts at 30 µg

Lane 3:

Unboiled HepG2 whole cell extracts at 30 µg

Lane 4:

Unboiled Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/1000 dilution

Lane 1:

Unboiled HepG2 whole cell extracts at 30 µg

Lane 2:

Unboiled Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/5000 dilution

Lanes 1 and 3:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled FZD4 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled FZD9 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged FZD4 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged FZD4 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2824

Isotype

IgG

Carrier free

Yes

Reacts with

Zebrafish, Human

Applications

applications

Immunogen

Synthetic Peptide within Human Frizzled-4.

Q9ULV1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for Wnt proteins (PubMed : 30135577). Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed : 30135577). Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

See full target information Frizzled-4

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Product promise

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