AB14475

Anti-Frizzled 5 antibody

(2 Reviews)

(6 Publications)

Rabbit Polyclonal Frizzled 5 antibody. Suitable for WB, IHC-FoFr, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

C2orf31, FZD5, Frizzled-5, Fz-5, hFz5, FzE5

1 Images

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Western blot - Anti-Frizzled 5 antibody (AB14475)

TENT lysates of transiently transfected 293 cells were probed with anti-Fz5 (1 g/ml). TENT lysing buffer; 20mM Tris-HCl, pH 8.0, 2mM EDTA, 150mM NaCl, and 1% Triton X-100; 1mM PMSF; 1ug/ml each aprotinin, leupeptin, pepstatin; 1mM Na3VO4; 1mM NaF

All lanes:

Western blot - Anti-Frizzled 5 antibody (ab14475)

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

WB, IHC-FoFr, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FZD5 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13467

Specificity

Recognizes human Fz5.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.609% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled 5 also known as FZD5 is a receptor that functions mechanically as a part of the Wnt signaling pathway. The Frizzled 5 protein has a molecular weight of around 75 kDa and is expressed in various tissues including the brain the eyes and the cardiovascular system. As a transmembrane receptor Frizzled 5 binds to Wnt ligands and activates intracellular signaling cascades mediating important cellular processes.

Biological function summary

Frizzled 5 participates in cellular communication and is part of the Wnt-frizzled protein complex. This complex plays a role in embryonic development cell proliferation and cell differentiation. Through its interaction with Wnt ligands Frizzled 5 can influence cell fate determination and contribute to the maintenance of stem cells. These activities are essential for development and tissue homeostasis.

Pathways

Knowledge of Frizzled 5 places it within both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway Frizzled 5 associates with LRP5/6 co-receptors to stabilize β-catenin leading to gene transcription changes. In the non-canonical pathway it involves the activation of Ca2+ signaling. Proteins related to Frizzled 5 in these pathways include Dishevelled (DVL) and the Glycogen synthase kinase (GSK-3).

Research has linked Frizzled 5 to conditions like retinopathy of prematurity and colorectal cancer. In retinopathy Frizzled 5's interaction with Norrin—a protein related to eye vascular development—can lead to abnormal vascularization. In colorectal cancer alterations in the Wnt-Frizzled signaling are implicated with β-catenin accumulating as a consequence. The modification in these signaling pathways hints at the potential for therapeutic interventions targeting Frizzled 5.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for Wnt proteins (PubMed : 10097073, PubMed : 20530549, PubMed : 26908622, PubMed : 9054360). Functions in the canonical Wnt/beta-catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (By similarity). In neurons, activation by WNT7A promotes formation of synapses (PubMed : 20530549). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity). Plays a role in ocular development (PubMed : 26908622).

See full target information FZD5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 56:3522-3537 PubMed30143969

2018

Silencing of Long Noncoding RNA SOX21-AS1 Relieves Neuronal Oxidative Stress Injury in Mice with Alzheimer's Disease by Upregulating FZD3/5 via the Wnt Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Yu Fang,Xuan Cheng,Ya-Jun Lian,Hong-Liang Xu

Cell adhesion & migration 11:24-38 PubMed27159116

2016

Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Skaria,Esther Bachli,Gabriele Schoedon

PloS one 9:e91086 PubMed24755605

2014

Wnt signaling in form deprivation myopia of the mice retina.

Applications

Species

Mouse

Mingming Ma,Zhengwei Zhang,Ergang Du,Wenjing Zheng,Qing Gu,Xun Xu,Bilian Ke

Nature communications 4:2803 PubMed24335906

2013

Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongde Ye,Chunxia Zhang,Tao Tu,Min Sun,Dan Liu,Di Lu,Jing Feng,Dongling Yang,Feng Liu,Xiyun Yan

Development (Cambridge, England) 137:2215-25 PubMed20530549

2010

Frizzled-5, a receptor for the synaptic organizer Wnt7a, regulates activity-mediated synaptogenesis.

Applications

ICC/IF, IHC-Fr, IHC-Fr, ICC/IF

Species

Mouse, Mouse, Rat, Rat

Macarena Sahores,Alasdair Gibb,Patricia C Salinas

Arteriosclerosis, thrombosis, and vascular biology 28:504-10 PubMed18174455

2008

Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Pereira,Dominik J Schaer,Esther B Bachli,Michael O Kurrer,Gabriele Schoedon

View all publications

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Product promise

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Anti-Frizzled 5 antibody

Western blot - Anti-Frizzled 5 antibody (AB14475)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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