Rabbit Polyclonal Frizzled 5 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human FZD5.
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- American journal of translational research 15:4727-47342023Study on the protective effect of chondroitin sulfate from sturgeons on rat chondrocytes and its potential mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Zhang,Qingsong Li,Lei ChenPubMed 37560250
- International journal of biological sciences 19:1123-11452023Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian ZhuPubMed 36923940
- Clinical, cosmetic and investigational dermatology 14:1651-16562021The Levels of Wnt5a and Its Receptors Frizzled5 and Frizzled2 as Immunohistochemical Biomarkers of Severity of Psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoying Ning,Dingwei Zhang,Yuan Wang,Jia Huo,Ying Huang,Ying Guo,Zhengxiao Li,Yanfei ZhangPubMed 34785924
- Neuron 109:3104-3118.e62021Oligodendroglial ring finger protein Rnf43 is an essential injury-specific regulator of oligodendrocyte maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianqin Niu,Guangdan Yu,Xiaorui Wang,Wenlong Xia,Yuxin Wang,Kimberly K Hoi,Feng Mei,Lan Xiao,Jonah R Chan,Stephen P J FancyPubMed 34390652
- Central-European journal of immunology 46:162-1822021CircNPM1 strengthens Adriamycin resistance in acute myeloid leukemia by mediating the miR-345-5p/FZD5 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Ding,Xiaochun Zhang,Jianan Xue,Le Fang,Chunmei Ban,Bin Song,Lili WuPubMed 34764785
- Cell death & disease 11:10602020FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Sun et. al.PubMed 33311446
- eLife 9:2020MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanella Prodromidou et. al.PubMed 32459171
- Molecular neurobiology 56:6777-67912019Wnt Signaling Alterations in the Human Spinal Cord of Amyotrophic Lateral Sclerosis Cases: Spotlight on Fz2 and Wnt5a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos González-Fernández et. al.PubMed 30924074
- Angiogenesis 20:615-6282017Frizzled-5: a high affinity receptor for secreted frizzled-related protein-2 activation of nuclear factor of activated T-cells c3 signaling to promote angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuri K Peterson et. al.PubMed 28840375
- Oncotarget 8:15168-151812017Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Sun,Suoning Liu,Daguang Wang,Yang Zhang,Wei Li,Yuchen Guo,Hua Zhang,Jian SuoPubMed 28147310
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FZD5. The exact immunogen used to generate this antibody is proprietary information. Database link Q13467
Reactivity data
Select an application| IHC-P | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for Wnt proteins (PubMed:10097073, PubMed:20530549, PubMed:26908622, PubMed:9054360). Functions in the canonical Wnt/beta-catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (By similarity). In neurons, activation by WNT7A promotes formation of synapses (PubMed:20530549). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity). Plays a role in ocular development (PubMed:26908622).
Alternative names
C2orf31, FZD5, Frizzled-5, Fz-5, hFz5, FzE5
Recommended products
Rabbit Polyclonal Frizzled 5 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human FZD5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FZD5. The exact immunogen used to generate this antibody is proprietary information. Database link Q13467
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Frizzled 5 also known as FZD5 is a receptor that functions mechanically as a part of the Wnt signaling pathway. The Frizzled 5 protein has a molecular weight of around 75 kDa and is expressed in various tissues including the brain the eyes and the cardiovascular system. As a transmembrane receptor Frizzled 5 binds to Wnt ligands and activates intracellular signaling cascades mediating important cellular processes.
- Biological function summary
Frizzled 5 participates in cellular communication and is part of the Wnt-frizzled protein complex. This complex plays a role in embryonic development cell proliferation and cell differentiation. Through its interaction with Wnt ligands Frizzled 5 can influence cell fate determination and contribute to the maintenance of stem cells. These activities are essential for development and tissue homeostasis.
- Pathways
Knowledge of Frizzled 5 places it within both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway Frizzled 5 associates with LRP5/6 co-receptors to stabilize β-catenin leading to gene transcription changes. In the non-canonical pathway it involves the activation of Ca2+ signaling. Proteins related to Frizzled 5 in these pathways include Dishevelled (DVL) and the Glycogen synthase kinase (GSK-3).
- Associated diseases and disorders
Research has linked Frizzled 5 to conditions like retinopathy of prematurity and colorectal cancer. In retinopathy Frizzled 5's interaction with Norrin—a protein related to eye vascular development—can lead to abnormal vascularization. In colorectal cancer alterations in the Wnt-Frizzled signaling are implicated with β-catenin accumulating as a consequence. The modification in these signaling pathways hints at the potential for therapeutic interventions targeting Frizzled 5.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-Frizzled 5 antibody (ab75234)
All lanes: Western blot - Anti-Frizzled 5 antibody (ab75234) at 1/500 dilution
Lane 1: LOVO cell extracts at 30 µg
Lane 2: LOVO cell extracts at 30 µg with immunising peptide
Predicted band size: 65 kDa
Observed band size: 48 kDa, 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Frizzled 5 antibody (ab75234)
ab75234 at 1/500 dilution staining Frizzled 5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 5 antibody (ab75234)
ab75234 (4µg/ml) staining Frizzled 5 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the Islets of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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