AB75234

Anti-Frizzled 5 antibody

Name: Anti-Frizzled 5 antibody (ab75234) | Abcam
Brand: Abcam Limited
SKU: AB75234
Price: 545 USD
Availability: InStock

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(25 Publications)

Rabbit Polyclonal Frizzled 5 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human FZD5.

View Alternative Names

C2orf31, FZD5, Frizzled-5, Fz-5, hFz5, FzE5

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 5 antibody (AB75234)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 5 antibody (AB75234)

ab75234 (4µg/ml) staining Frizzled 5 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the Islets of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Frizzled 5 antibody (AB75234)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Frizzled 5 antibody (AB75234)

ab75234 at 1/500 dilution staining Frizzled 5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

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Western blot - Anti-Frizzled 5 antibody (AB75234)

All lanes:

Western blot - Anti-Frizzled 5 antibody (ab75234) at 1/500 dilution

Lane 1:

LOVO cell extracts at 30 µg

Lane 2:

LOVO cell extracts at 30 µg with immunising peptide

Predicted band size: 65 kDa

Observed band size: 48 kDa,65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, WB, ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human FZD5. The exact immunogen used to generate this antibody is proprietary information.

Q13467

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled 5 also known as FZD5 is a receptor that functions mechanically as a part of the Wnt signaling pathway. The Frizzled 5 protein has a molecular weight of around 75 kDa and is expressed in various tissues including the brain the eyes and the cardiovascular system. As a transmembrane receptor Frizzled 5 binds to Wnt ligands and activates intracellular signaling cascades mediating important cellular processes.

Biological function summary

Frizzled 5 participates in cellular communication and is part of the Wnt-frizzled protein complex. This complex plays a role in embryonic development cell proliferation and cell differentiation. Through its interaction with Wnt ligands Frizzled 5 can influence cell fate determination and contribute to the maintenance of stem cells. These activities are essential for development and tissue homeostasis.

Pathways

Knowledge of Frizzled 5 places it within both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway Frizzled 5 associates with LRP5/6 co-receptors to stabilize β-catenin leading to gene transcription changes. In the non-canonical pathway it involves the activation of Ca2+ signaling. Proteins related to Frizzled 5 in these pathways include Dishevelled (DVL) and the Glycogen synthase kinase (GSK-3).

Research has linked Frizzled 5 to conditions like retinopathy of prematurity and colorectal cancer. In retinopathy Frizzled 5's interaction with Norrin—a protein related to eye vascular development—can lead to abnormal vascularization. In colorectal cancer alterations in the Wnt-Frizzled signaling are implicated with β-catenin accumulating as a consequence. The modification in these signaling pathways hints at the potential for therapeutic interventions targeting Frizzled 5.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for Wnt proteins (PubMed : 10097073, PubMed : 20530549, PubMed : 26908622, PubMed : 9054360). Functions in the canonical Wnt/beta-catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (By similarity). In neurons, activation by WNT7A promotes formation of synapses (PubMed : 20530549). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity). Plays a role in ocular development (PubMed : 26908622).

See full target information FZD5

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1461546 PubMed39735608

2024

MAFG-DT promotes prostate cancer bone metastasis through activation of the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chongwen Wang,Zheng Zhou,Yongjie Ye,Liqiang Zhou,Jialun Wang,Zhi Zhang

Cells 13: PubMed38891118

2024

Ileal Crohn's Disease Exhibits Reduced Activity of Phospholipase C-β3-Dependent Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoaki Ando,Ikuo Takazawa,Zachary T Spencer,Ryoji Ito,Yoshiaki Tomimori,Zbigniew Mikulski,Kenji Matsumoto,Tohru Ishitani,Lee A Denson,Yu Kawakami,Yuko Kawakami,Jiro Kitaura,Yashi Ahmed,Toshiaki Kawakami

Journal of biochemical and molecular toxicology 38:e23742 PubMed38780005

2024

circ_0000592 facilitates the progression of esophageal squamous cell carcinoma via miR-155-5p/FZD5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxian He,Kaizhong Yu,Gaofeng Liang,Weiyu Shen,Hui Tian

Heliyon 10:e29733 PubMed38707304

2024

DNMT3A-mediated DNA methylation and transcription inhibition of FZD5 suppresses lung carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiqiao Shen,Jinchen Su,Xiangling Chu,Xin Yu,Xiwen Gao

American journal of translational research 15:4727-4734 PubMed37560250

2023

Study on the protective effect of chondroitin sulfate from sturgeons on rat chondrocytes and its potential mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhang,Qingsong Li,Lei Chen

International journal of biological sciences 19:1123-1145 PubMed36923940

2023

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian Zhu

Clinical, cosmetic and investigational dermatology 14:1651-1656 PubMed34785924

2021

The Levels of Wnt5a and Its Receptors Frizzled5 and Frizzled2 as Immunohistochemical Biomarkers of Severity of Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Ning,Dingwei Zhang,Yuan Wang,Jia Huo,Ying Huang,Ying Guo,Zhengxiao Li,Yanfei Zhang

Neuron 109:3104-3118.e6 PubMed34390652

2021

Oligodendroglial ring finger protein Rnf43 is an essential injury-specific regulator of oligodendrocyte maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqin Niu,Guangdan Yu,Xiaorui Wang,Wenlong Xia,Yuxin Wang,Kimberly K Hoi,Feng Mei,Lan Xiao,Jonah R Chan,Stephen P J Fancy

Central-European journal of immunology 46:162-182 PubMed34764785

2021

CircNPM1 strengthens Adriamycin resistance in acute myeloid leukemia by mediating the miR-345-5p/FZD5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ding,Xiaochun Zhang,Jianan Xue,Le Fang,Chunmei Ban,Bin Song,Lili Wu

Cell death & disease 11:1060 PubMed33311446

2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Zhuo Wang,Lei Na,Dan Dong,Wei Wang,Chenghai Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Frizzled 5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 5 antibody (AB75234)

Immunocytochemistry/ Immunofluorescence - Anti-Frizzled 5 antibody (AB75234)

Western blot - Anti-Frizzled 5 antibody (AB75234)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

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