AB155093

Anti-Frizzled 8 antibody [EPR7308(2)]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Frizzled 8 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Frizzled-8, Fz-8, hFz8, FZD8

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin embedded human normal pancreas tissue using ab155093 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Frizzled 8 with Purified ab155093 at 1 : 500 dilution (1.17 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

WB

Unknown

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

Primary antibody diluted with 1% BSA.

All lanes:

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 73 kDa

false

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

WB

Lab

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

All lanes:

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

Human breast cancer lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

WB

Lab

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (AB155093)

The expression level of Frizzled 8 in normal tissues is low and can be upregulated in cancer tissues reported by PMID 23815780 and 22138402

All lanes:

Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

Lane 1:

Human prostate cancer lysates at 15 µg

Lane 2:

Human prostate lysates at 15 µg

Lane 3:

Human lung cancer lysates at 15 µg

Lane 4:

Human lung lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

false

Carrier free

Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7308(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

HeLa and Jurkat lysates; Human endometrial carcinoma tissue.

Specificity

The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovations Ltd. Rhodadyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled 8 also known as FZD8 forms part of the Frizzled family of proteins which serve as receptors for the Wnt signaling pathway. This protein with a mass of approximately 70 kDa contains a characteristic cysteine-rich domain. FZD8 mainly localizes to the cell membrane and prominently expresses in tissues such as the brain heart and placenta. Its structure includes seven transmembrane domains which contributes significantly to its role in signal transduction.

Biological function summary

FZD8 influences cellular communication within the Wnt signaling pathway impacting processes like cell proliferation and fate determination. FZD8 often associates with other proteins in complex formations particularly when assembling with Wnt ligands on the cell surface. It plays an important role in embryonic development and tissue homeostasis where precise cellular signaling is necessary for maintaining the balance between cell growth and programmed cell death.

Pathways

FZD8 interacts most prominently within the canonical Wnt/β-catenin signaling pathway. It works closely with the Dishevelled (DVL) protein facilitating signal transfer from the cell surface to the cytoplasm and beyond. This involvement tightly links FZD8 to many developmental processes and cellular communication networks. Additionally FZD8 is also implicated in the non-canonical Wnt pathways demonstrating its versatility in various cellular signaling contexts.

FZD8 is associated with conditions such as cancer and neurodegenerative diseases. Dysregulation of FZD8 may contribute to abnormal cell proliferation as observed in certain cancers when improper Wnt signaling causes uncontrolled cellular growth. Additionally its connection with Alzheimer's disease highlights the importance of FZD8 in neuronal maintenance and function. Abberations in FZD8 or its signaling partners such as β-catenin may lead to pathogenesis in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

See full target information FZD8

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 84:3235-3249 PubMed39024552

2024

CCN6 Suppresses Metaplastic Breast Carcinoma by Antagonizing Wnt/β-Catenin Signaling to Inhibit EZH2-Driven EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Gonzalez,Bryce Brophy,Ahmad Eido,Adele E Leonetti,Sabra I Djomehri,Giuseppina Augimeri,Nicholas J Carruthers,Raymond G Cavalcante,Francesca Giordano,Sebastiano Andò,Alexey I Nesvizhskii,Eric R Fearon,Celina G Kleer

International journal of molecular sciences 25: PubMed38928134

2024

Evaluation of the Canonical Wnt Signaling Pathway in the Hearts of Hypertensive Rats of Various Etiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Maryla Anna Młynarczyk,Natalia Domian,Irena Kasacka

Journal of the Egyptian National Cancer Institute 36:8 PubMed38494582

2024

CircMCTP2 enhances the progression of bladder cancer by regulating the miR-99a-5p/FZD8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Kexin Zhang,Xianxu Yang

Oncology letters 26:477 PubMed37809047

2023

Expression pattern, prognostic value and potential microRNA silencing of FZD8 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam H Al-Zahrani,Mourad Assidi,Peter Natesan Pushparaj,Jaudah Al-Maghrabi,Ali Zari,Atlal Abusanad,Abdelbaset Buhmeida,Muhammad Abu-Elmagd

Oncogene 42:3062-3074 PubMed37634009

2023

WNT2-SOX4 positive feedback loop promotes chemoresistance and tumorigenesis by inducing stem-cell like properties in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Yu Tan,Yu-Ting Li,Hua-Hui Li,Li-Xiang Ma,Chui-Mian Zeng,Tian-Tian Zhang,Tu-Xiong Huang,Xiao-Di Zhao,Li Fu

Clinical and experimental pharmacology & physiology 50:19-27 PubMed36047789

2022

Angiotensin-receptor blocker losartan alleviates atrial fibrillation in rats by downregulating frizzled 8 and inhibiting the activation of WNT-5A pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ronghua Feng,Jinjie Wan,Yongsheng He,Hui Gong,Zeqin Xu,Jiugeng Feng

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21883 PubMed34569659

2021

Mechanosensitive miR-100 coordinates TGFβ and Wnt signaling in osteocytes during fluid shear stress.

Applications

Unspecified application

Species

Unspecified reactive species

Neha S Dole,Jihee Yoon,David A Monteiro,Jason Yang,Courtney M Mazur,Serra Kaya,Cassandra D Belair,Tamara Alliston

OncoTargets and therapy 12:2615-2621 PubMed31040702

2019

miRNA-99b-5p targets FZD8 to inhibit non-small cell lung cancer proliferation, migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Yajuan Chen,Tao Shou,Jing Hu,Chen Qing

Oncology research 25:1297-1304 PubMed28247840

2017

MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wang,Wenquan Pang,Zhenbai Zuo,Wenyan Zhang,Weidong He

View all publications

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Product promise

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