Rabbit Polyclonal FRMD4A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FRMD4A aa 900-1000.
Images
Publications
Filter by
- Experimental and therapeutic medicine 11:421-4262016Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongtian Fan,Dechuan Li,Jun Qian,Yong Liu,Haiyang Feng,Dechuan LiPubMed 26893625
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FRMD4A aa 900-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2Q2
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex (By similarity). Plays a redundant role with FRMD4B in epithelial polarization (By similarity). May regulate MAPT secretion by activating ARF6-signaling (PubMed:27044754).
Alternative names
FRMD4, KIAA1294, FRMD4A, FERM domain-containing protein 4A
Recommended products
Rabbit Polyclonal FRMD4A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FRMD4A aa 900-1000.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FRMD4A aa 900-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2Q2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FRMD4A also known as FERM Domain Containing 4A is a protein with an important role in cellular signaling. It has a molecular mass of approximately 80 kDa. This protein is expressed in various human tissues with strong presence in the brain. As a part of the FERM domain-containing protein family FRMD4A participates in the organization of the cell cortex and binds to several partners facilitating signal transduction within cells.
- Biological function summary
FRMD4A influences several intracellular mechanisms. It associates with a protein complex involved in cellular adhesion and junctions. This protein supports the maintenance of cell polarity and potentially regulates the cytoskeleton. The interaction of FRMD4A with cytohesin proteins notably showcases its potential role in modulating cellular activities like migration and morphogenesis.
- Pathways
FRMD4A plays an active role in the Epithelial Growth Factor Receptor (EGFR) signaling pathway and cell adhesion pathways. It frequently interacts with cytohesin-1 linking it to critical intracellular signaling processes. These interactions suggest that FRMD4A contributes to the regulation of signaling pathways that coordinate cell growth and structural organization.
- Associated diseases and disorders
FRMD4A associates with neurological conditions like schizophrenia and autism spectrum disorders. Researchers have identified potential links between FRMD4A and disease through its interaction with proteins such as DISC1 a well-known genetic risk factor for psychiatric illnesses. These connections highlight FRMD4A as a possible target for therapeutic research in neurodevelopmental and neuropsychiatric conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (ab122475)
ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human lymphoid tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-FRMD4A antibody (ab122475)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (ab122475)
ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (ab122475)
ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (ab122475)
ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com