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AB122475

Anti-FRMD4A antibody

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(1 Publication)

Rabbit Polyclonal FRMD4A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FRMD4A aa 900-1000.

View Alternative Names

FRMD4, KIAA1294, FRMD4A, FERM domain-containing protein 4A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-FRMD4A antibody (AB122475)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FRMD4A antibody (AB122475)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)

ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)

ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human lymphoid tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)

ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4A antibody (AB122475)

ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FRMD4A aa 900-1000. The exact immunogen used to generate this antibody is proprietary information.

Q9P2Q2

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Sample Prep & Detection Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRMD4A also known as FERM Domain Containing 4A is a protein with an important role in cellular signaling. It has a molecular mass of approximately 80 kDa. This protein is expressed in various human tissues with strong presence in the brain. As a part of the FERM domain-containing protein family FRMD4A participates in the organization of the cell cortex and binds to several partners facilitating signal transduction within cells.
Biological function summary

FRMD4A influences several intracellular mechanisms. It associates with a protein complex involved in cellular adhesion and junctions. This protein supports the maintenance of cell polarity and potentially regulates the cytoskeleton. The interaction of FRMD4A with cytohesin proteins notably showcases its potential role in modulating cellular activities like migration and morphogenesis.

Pathways

FRMD4A plays an active role in the Epithelial Growth Factor Receptor (EGFR) signaling pathway and cell adhesion pathways. It frequently interacts with cytohesin-1 linking it to critical intracellular signaling processes. These interactions suggest that FRMD4A contributes to the regulation of signaling pathways that coordinate cell growth and structural organization.

FRMD4A associates with neurological conditions like schizophrenia and autism spectrum disorders. Researchers have identified potential links between FRMD4A and disease through its interaction with proteins such as DISC1 a well-known genetic risk factor for psychiatric illnesses. These connections highlight FRMD4A as a possible target for therapeutic research in neurodevelopmental and neuropsychiatric conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex (By similarity). Plays a redundant role with FRMD4B in epithelial polarization (By similarity). May regulate MAPT secretion by activating ARF6-signaling (PubMed : 27044754).
See full target information FRMD4A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 11:421-426 PubMed26893625

2016

Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Fan,Dechuan Li,Jun Qian,Yong Liu,Haiyang Feng,Dechuan Li
View all publications
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Product promise

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