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AB249649

Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FRMD6/Willin antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C14orf31, FRMD6, FERM domain-containing protein 6, Willin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)

This data was developed using ab171745, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A431 cells labeling FRMD6/Willin using ab171745 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)

This data was developed using ab171745, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized A431 cells labeling FRMD6/Willin using ab171745 (red) at 1/100 dilution or a rabbit IgG (negative) (green).

Western blot - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)
  • WB

Supplier Data

Western blot - Anti-FRMD6/Willin antibody [EPR12261] - BSA and Azide free (AB249649)

This data was developed using ab171745, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FRMD6/Willin antibody [EPR12261] (<a href='/en-us/products/primary-antibodies/frmd6-willin-antibody-epr12261-ab171745'>ab171745</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Predicted band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12261

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249649 is the carrier-free version of ab171745.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRMD6 also known as Willin is a protein that functions by acting as a scaffold within cells influencing cell shape and motility. This protein has an approximate mass of 78 kDa. FRMD6 is expressed in multiple tissues including the brain with noticeable presence in neuronal cells. It plays a role in the organization of actin cytoskeleton impacting cell adhesion and polarity.
Biological function summary

FRMD6 influences signaling pathways that regulate growth and differentiation. It contributes as part of a regulatory complex that communicates signals from the extracellular matrix to intracellular pathways. The protein is not known to be part of a stable complex but it interacts dynamically with several cytoskeletal elements during cell signaling events. It assists in transducing signals necessary for maintaining cellular architecture and function.

Pathways

FRMD6 is significantly involved in the Hippo signaling pathway which regulates cell proliferation and apoptosis. This pathway controls tissue growth and cancer development through interaction with proteins like YAP and TAZ. FRMD6 operates upstream within this pathway contributing to the suppression of cell growth by modulating these transcriptional coactivators.

FRMD6 associates with diverse pathological conditions. In cancer aberrant expression or function of FRMD6 interrupts normal Hippo pathway signaling potentially promoting tumorigenesis. Additionally studies have linked FRMD6 with neurological disorders reflecting its expression in neuronal tissues. It connects with proteins like LATS1 and MOB1A in these disorders influencing their roles within the affected pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information FRMD6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 52:e70032 PubMed40069996

2025

Pancreatitis Independently Induced by Hypertriglyceridaemia Outlines the Immune Profiles of HTGP in Clinic.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxing Liu,Caixia Liang,Wenjing Yan,Yanfeng Zhang,Yi Pan,Yue Yang,Fangfang Zhang,Xiaojian Liu,Liang Jin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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