AB183492

Anti-FRS2 antibody [EPR14724]

RabMAb
Recombinant
What is this?

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(9 Publications)

Rabbit Recombinant Monoclonal FRS2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1, SNT-1, FRS2

4 Images

Flow Cytometry (Intracellular) - Anti-FRS2 antibody [EPR14724] (AB183492)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FRS2 antibody [EPR14724] (AB183492)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedSH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to aRabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-FRS2 antibody [EPR14724] (AB183492)

All lanes:

Western blot - Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 57 kDa

false

Supplier Data

Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (AB183492)

Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of Human fetal brain lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (ab183492)

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-FRS2 antibody [EPR14724] (AB183492)

All lanes:

Western blot - Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution

All lanes:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 57 kDa

false

Carrier free

Anti-FRS2 antibody [EPR14724] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14724

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRS2 also known as Fibroblast growth factor receptor substrate 2 functions as an adaptor protein in cellular signal transduction. This protein with a molecular mass of approximately 60 kDa plays a critical role in linking receptor tyrosine kinases specifically FGFR (Fibroblast Growth Factor Receptor) to downstream signaling pathways. FRS2 primarily expresses in various tissues with notable expression in neural and epithelial cells. Its function involves phosphorylation and the recruitment of downstream signaling molecules which propagate cellular response signals.

Biological function summary

FRS2 integrates into several signaling complexes that regulate key cellular processes. It acts as a scaffold protein creating platforms for the assembly of signaling complexes such as those involving Grb2 (Growth factor receptor-bound protein 2) and Sos (Son of Sevenless). These complexes mediate processes related to cell differentiation proliferation and survival. The protein's ability to anchor and direct these signaling molecules highlights its importance within cellular mechanisms where it influences signaling cascades leading to specific cellular outcomes.

Pathways

The role of FRS2 is essential in the FGFR and MAPK/ERK pathways. Within these pathways FRS2 interacts closely with proteins like FGFR facilitating their activation and consequent downstream signaling events. Through these interactions the protein influences pathways involved in developmental processes and cellular maintenance. FRS2 by enabling FGFR signal transduction significantly impacts cellular responses to external growth stimuli making it an important player in normal cellular function and development.

Abnormalities in FRS2 function or expression link to certain pathologies including cancer and neurological disorders. For instance overactivation of FRS2-mediated signaling pathways can contribute to oncogenic processes leading to uncontrolled cell growth and proliferation. Furthermore mutations affecting FRS2 interaction with FGFR have implications in developmental disorders characterized by neural defects. Understanding FRS2 connections in these diseases helps in identifying potential therapeutic targets for treatment interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

See full target information FRS2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40014401

2025

Identification of potent biparatopic antibodies targeting FGFR2 fusion-driven cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Saireudee Chaturantabut,Sydney Oliver,Dennie T Frederick,Jiwan J Kim,Foxy P Robinson,Alessandro Sinopoli,Tian-Yu Song,Yao He,Yuan-Chen Chang,Diego J Rodriguez,Liang Chang,Devishi Kesar,Meilani Ching,Ruvimbo Dzvurumi,Adel Atari,Yuen-Yi Tseng,Nabeel Bardeesy,William R Sellers

International journal of molecular sciences 24: PubMed37569547

2023

Knockdown of PTK7 Reduces the Oncogenic Potential of Breast Cancer Cells by Impeding Receptor Tyrosine Kinase Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Won-Sik Shin,Si Won Oh,Han Na Park,Jae Hoon Kim,Seung-Taek Lee

The Journal of biological chemistry 299:102729 PubMed36410439

2022

Systematic pharmacological analysis of agonistic and antagonistic fibroblast growth factor receptor 1 MAbs reveals a similar unique mode of action.

Applications

Unspecified application

Species

Unspecified reactive species

Jocelyn Chan,Joyce Chan,Lily Shao,Scott S Stawicki,Victoria C Pham,Rob W Akita,Marc Hafner,Lisa Crocker,Kebing Yu,James T Koerber,Gabriele Schaefer,Laetitia Comps-Agrar

Evidence-based complementary and alternative medicine : eCAM 2022:6235771 PubMed36387361

2022

TRIM44 Promotes Endometrial Carcinoma Progression by Activating the FRS2 Signalling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Song,Guiju Zhou,Enxue Song,Lei Zhan,Qingyuan Wang,Hui Song,Jingxian Xia,Lin Cong

Journal of oncology 2022:4537343 PubMed36304988

2022

Long Noncoding RNA XIST Promotes Resistance to Lenvatinib in Hepatocellular Carcinoma Cells via Epigenetic Inhibition of NOD2.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Duan,Hui Li,Wenlong Yu,Yongjie Zhang,Lei Yin

British journal of cancer 127:1239-1253 PubMed35864158

2022

LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Xie,Kunpeng Du,Wei Liu,Chunshan Liu,Baiyao Wang,Yunhong Tian,Rong Li,Xiaoting Huang,Jie Lin,Haifeng Jian,Jian Zhang,Yawei Yuan

Cancer discovery 12:1378-1395 PubMed35420673

2022

EGFR Inhibition Potentiates FGFR Inhibitor Therapy and Overcomes Resistance in FGFR2 Fusion-Positive Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qibiao Wu,Yuanli Zhen,Lei Shi,Phuong Vu,Patricia Greninger,Ramzi Adil,Joshua Merritt,Regina Egan,Meng-Ju Wu,Xunqin Yin,Cristina R Ferrone,Vikram Deshpande,Islam Baiev,Christopher J Pinto,Daniel E McLoughlin,Charlotte S Walmsley,James R Stone,John D Gordan,Andrew X Zhu,Dejan Juric,Lipika Goyal,Cyril H Benes,Nabeel Bardeesy

Cancers 12: PubMed33092134

2020

Novel Therapeutic Insights in Dedifferentiated Liposarcoma: A Role for FGFR and MDM2 Dual Targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Bérengère Dadone-Montaudié,Audrey Laroche-Clary,Aline Mongis,Emmanuel Chamorey,Ilaria Di Mauro,Vanessa Chaire,Pascal Finetti,Renaud Schiappa,François Le Loarer,Isabelle Birtwisle-Peyrottes,Jean-François Michiels,François Bertucci,Florence Pedeutour,Antoine Italiano,Laurence Bianchini

Cancer discovery 9:1064-1079 PubMed31109923

2019

TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion-Positive Intrahepatic Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lipika Goyal,Lei Shi,Leah Y Liu,Ferran Fece de la Cruz,Jochen K Lennerz,Srivatsan Raghavan,Ignaty Leschiner,Liudmila Elagina,Giulia Siravegna,Raymond W S Ng,Phuong Vu,Krushna C Patra,Supriya K Saha,Raul N Uppot,Ron Arellano,Stephanie Reyes,Takeshi Sagara,Sachie Otsuki,Brandon Nadres,Heather A Shahzade,Ipsita Dey-Guha,Isobel J Fetter,Islam Baiev,Emily E Van Seventer,Janet E Murphy,Cristina R Ferrone,Kenneth K Tanabe,Vikram Deshpande,James J Harding,Rona Yaeger,Robin K Kelley,Alberto Bardelli,A John Iafrate,William C Hahn,Cyril H Benes,David T Ting,Hiroshi Hirai,Gad Getz,Dejan Juric,Andrew X Zhu,Ryan B Corcoran,Nabeel Bardeesy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FRS2 antibody [EPR14724]

Flow Cytometry (Intracellular) - Anti-FRS2 antibody [EPR14724] (AB183492)

Western blot - Anti-FRS2 antibody [EPR14724] (AB183492)

Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (AB183492)

Western blot - Anti-FRS2 antibody [EPR14724] (AB183492)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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