Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (AB320728), expandable thumbnail](https://content.abcam.com/products/images/frs2-antibody-epr28714-126-bsa-and-azide-free-ab320728--western-blot-img435540.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (AB320728)")
![Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (AB320728), expandable thumbnail](https://content.abcam.com/products/images/frs2-antibody-epr28714-126-bsa-and-azide-free-ab320728--western-blot-img435539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (AB320728)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Mouse | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Alternative names
Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, FRS2-alpha, Suc1-associated neurotrophic factor target 1, SNT-1
Recommended products
Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Alternative names
Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, FRS2-alpha, Suc1-associated neurotrophic factor target 1, SNT-1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28714-126
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab320728 is the carrier-free version of Anti-FRS2 antibody [EPR28714-126] ab320727.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
FRS2 also known as Fibroblast growth factor receptor substrate 2 functions as an adaptor protein in cellular signal transduction. This protein with a molecular mass of approximately 60 kDa plays a critical role in linking receptor tyrosine kinases specifically FGFR (Fibroblast Growth Factor Receptor) to downstream signaling pathways. FRS2 primarily expresses in various tissues with notable expression in neural and epithelial cells. Its function involves phosphorylation and the recruitment of downstream signaling molecules which propagate cellular response signals.
- Biological function summary
FRS2 integrates into several signaling complexes that regulate key cellular processes. It acts as a scaffold protein creating platforms for the assembly of signaling complexes such as those involving Grb2 (Growth factor receptor-bound protein 2) and Sos (Son of Sevenless). These complexes mediate processes related to cell differentiation proliferation and survival. The protein's ability to anchor and direct these signaling molecules highlights its importance within cellular mechanisms where it influences signaling cascades leading to specific cellular outcomes.
- Pathways
The role of FRS2 is essential in the FGFR and MAPK/ERK pathways. Within these pathways FRS2 interacts closely with proteins like FGFR facilitating their activation and consequent downstream signaling events. Through these interactions the protein influences pathways involved in developmental processes and cellular maintenance. FRS2 by enabling FGFR signal transduction significantly impacts cellular responses to external growth stimuli making it an important player in normal cellular function and development.
- Associated diseases and disorders
Abnormalities in FRS2 function or expression link to certain pathologies including cancer and neurological disorders. For instance overactivation of FRS2-mediated signaling pathways can contribute to oncogenic processes leading to uncontrolled cell growth and proliferation. Furthermore mutations affecting FRS2 interaction with FGFR have implications in developmental disorders characterized by neural defects. Understanding FRS2 connections in these diseases helps in identifying potential therapeutic targets for treatment interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728), expandable thumbnail](https://content.abcam.com/products/images/frs2-antibody-epr28714-126-bsa-and-azide-free-ab320728--western-blot-img435540.jpg "Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728)")
Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728)
This data was developed using Anti-FRS2 antibody [EPR28714-126] ab320727, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-FRS2 antibody [EPR28714-126] (Anti-FRS2 antibody [EPR28714-126] ab320727) at 1/1000 dilution
Lane 1: F9 (mouse embryonal carcinoma epithelial cell) non-membrane fraction at 20 µg
Lane 2: F9 (mouse embryonal carcinoma epithelial cell) membrane fraction at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 74 kDa, 116 kDa, 135 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5000 dilution.
![Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728), expandable thumbnail](https://content.abcam.com/products/images/frs2-antibody-epr28714-126-bsa-and-azide-free-ab320728--western-blot-img435539.jpg "Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728)")
Western blot - Anti-FRS2 antibody [EPR28714-126] - BSA and Azide free (ab320728)
This data was developed using Anti-FRS2 antibody [EPR28714-126] ab320727, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 15 seconds, lanes 3-5: 180 seconds
All lanes: Western blot - Anti-FRS2 antibody [EPR28714-126] (Anti-FRS2 antibody [EPR28714-126] ab320727) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: NIH/3T3 transfected with siRNA specifically targeting FRS2 whole cell lysate at 20 µg
Lane 3: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Mouse liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 74 kDa, 36 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands higher than 150 kDa and lower than 50 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 15 seconds, lanes 3-5: 180 seconds
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com