JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308575

Anti-FRS2 antibody [HL1774] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human FRS2.
1 Images
Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)
  • WB

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/5000 dilution

All lanes:

RT-4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57.029 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1774

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FRS2. The exact immunogen used to generate this antibody is proprietary information.

Q8WU20

style
left-column

Complementary Products

Assay Kits

AB322428

Human FRS2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FRS2 ELISA Kit (AB322428)

  • 0
  • 0
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Sample Prep & Detection Kits

AB269889

Europium Conjugation Kit

Dot Blot - Europium Conjugation Kit (AB269889)

  • 0
  • 0
Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

  • 5
  • 4
Primary Antibodies

AB59194

Anti-FGFR1 (phospho Y654) antibody

BOND RX™ Validated

Western blot - Anti-FGFR1 (phospho Y654) antibody (AB59194)

  • 4
  • 3
Primary Antibodies

AB40776

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB40776)

  • 0
  • 1
Primary Antibodies

AB201015

Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] (AB201015)

  • 4
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRS2 also known as Fibroblast growth factor receptor substrate 2 functions as an adaptor protein in cellular signal transduction. This protein with a molecular mass of approximately 60 kDa plays a critical role in linking receptor tyrosine kinases specifically FGFR (Fibroblast Growth Factor Receptor) to downstream signaling pathways. FRS2 primarily expresses in various tissues with notable expression in neural and epithelial cells. Its function involves phosphorylation and the recruitment of downstream signaling molecules which propagate cellular response signals.
Biological function summary

FRS2 integrates into several signaling complexes that regulate key cellular processes. It acts as a scaffold protein creating platforms for the assembly of signaling complexes such as those involving Grb2 (Growth factor receptor-bound protein 2) and Sos (Son of Sevenless). These complexes mediate processes related to cell differentiation proliferation and survival. The protein's ability to anchor and direct these signaling molecules highlights its importance within cellular mechanisms where it influences signaling cascades leading to specific cellular outcomes.

Pathways

The role of FRS2 is essential in the FGFR and MAPK/ERK pathways. Within these pathways FRS2 interacts closely with proteins like FGFR facilitating their activation and consequent downstream signaling events. Through these interactions the protein influences pathways involved in developmental processes and cellular maintenance. FRS2 by enabling FGFR signal transduction significantly impacts cellular responses to external growth stimuli making it an important player in normal cellular function and development.

Abnormalities in FRS2 function or expression link to certain pathologies including cancer and neurological disorders. For instance overactivation of FRS2-mediated signaling pathways can contribute to oncogenic processes leading to uncontrolled cell growth and proliferation. Furthermore mutations affecting FRS2 interaction with FGFR have implications in developmental disorders characterized by neural defects. Understanding FRS2 connections in these diseases helps in identifying potential therapeutic targets for treatment interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
See full target information FRS2

Alternative Names

Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1, SNT-1, FRS2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com