AB308575

Anti-FRS2 antibody [HL1774] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human FRS2.

View Alternative Names

Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1, SNT-1, FRS2

5 Images

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/50000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

Transfected 293T whole cell extract with FRS2 shRNA 1 at 30 µg

Lane 3:

Transfected 293T whole cell extract with FRS2 shRNA 2 at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/5000 dilution

All lanes:

RT-4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57.029 kDa

false

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody HL1774 (ab308575) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/5000 dilution

Lane 1:

MCF-7 lysate at 30 µg

Lane 2:

NIH-3T3 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57.03 kDa

false

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody HL1774 (ab308575) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/5000 dilution

Lane 1:

mouse cerebellum tissue lysate at 50 µg

Lane 2:

mouse pancreas tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57.03 kDa

false

Supplier Data

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody HL1774 (ab308575) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (ab308575) at 1/1000 dilution

Lane 1:

PC-12 cell lysate at 30 µg

Lane 2:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57.03 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1774

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human FRS2. The exact immunogen used to generate this antibody is proprietary information.

Q8WU20

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRS2 also known as Fibroblast growth factor receptor substrate 2 functions as an adaptor protein in cellular signal transduction. This protein with a molecular mass of approximately 60 kDa plays a critical role in linking receptor tyrosine kinases specifically FGFR (Fibroblast Growth Factor Receptor) to downstream signaling pathways. FRS2 primarily expresses in various tissues with notable expression in neural and epithelial cells. Its function involves phosphorylation and the recruitment of downstream signaling molecules which propagate cellular response signals.

Biological function summary

FRS2 integrates into several signaling complexes that regulate key cellular processes. It acts as a scaffold protein creating platforms for the assembly of signaling complexes such as those involving Grb2 (Growth factor receptor-bound protein 2) and Sos (Son of Sevenless). These complexes mediate processes related to cell differentiation proliferation and survival. The protein's ability to anchor and direct these signaling molecules highlights its importance within cellular mechanisms where it influences signaling cascades leading to specific cellular outcomes.

Pathways

The role of FRS2 is essential in the FGFR and MAPK/ERK pathways. Within these pathways FRS2 interacts closely with proteins like FGFR facilitating their activation and consequent downstream signaling events. Through these interactions the protein influences pathways involved in developmental processes and cellular maintenance. FRS2 by enabling FGFR signal transduction significantly impacts cellular responses to external growth stimuli making it an important player in normal cellular function and development.

Abnormalities in FRS2 function or expression link to certain pathologies including cancer and neurological disorders. For instance overactivation of FRS2-mediated signaling pathways can contribute to oncogenic processes leading to uncontrolled cell growth and proliferation. Furthermore mutations affecting FRS2 interaction with FGFR have implications in developmental disorders characterized by neural defects. Understanding FRS2 connections in these diseases helps in identifying potential therapeutic targets for treatment interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

See full target information FRS2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FRS2 antibody [HL1774] - BSA and Azide free

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Western blot - Anti-FRS2 antibody [HL1774] - BSA and Azide free (AB308575)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise