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AB317352

Anti-FRS2 + FRS3 antibody [EPR28555-60] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1, SNT-1, FRS2, Fibroblast growth factor receptor substrate 3, FGFR substrate 3, FGFR-signaling adaptor SNT2, Suc1-associated neurotrophic factor target 2, SNT-2, FRS3

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Sandwich ELISA - Anti-FRS2 + FRS3 antibody [EPR28555-60] - BSA and Azide free (Detector) (AB317352)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FRS2 + FRS3 antibody [EPR28555-60] - BSA and Azide free (Detector) (AB317352)

Sandwich ELISA of ab317350 with the capture antibody ab317351 dilution at 2 ug/mL and detector antibody ab317352 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28555-60

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab317352 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify FRS2. The recommended pair for sandwich ELISA is:

Capture: ab317351, FRS2 Capture Antibody (unconjugated)

Detector: ab317352, FRS2 + FSR3 Detector Antibody (unconjugated)

The reference range value is 19.53-2500 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FRS2 and FRS3 proteins also known as Fibroblast Growth Factor Receptor Substrate 2 and 3 serve as adaptor proteins in signal transduction processes. FRS2 with a mass of approximately 60 kDa and FRS3 play key roles in linking cell surface receptors to downstream signaling pathways. They are ubiquitously expressed but have higher concentrations in certain tissues like the brain and heart. These proteins have modular structures that contain phosphotyrosine-binding domains and SH2 domains which are involved in protein-protein interactions.
Biological function summary

The adaptor proteins FRS2 and FRS3 mediate signals from receptor tyrosine kinases notably the Fibroblast Growth Factor Receptors (FGFRs). Through their action they facilitate the assembly of signaling complexes necessary for cellular proliferation differentiation and survival. FRS2 and FRS3 activate important downstream effectors like the Ras/MAPK and PI3K/AKT pathways. They not only recognize activated receptors but also recruit other adaptors and signaling molecules to form complex networks.

Pathways

The proteins FRS2 and FRS3 are integral to the FGFR and ERK/MAPK signaling pathways. In these pathways they are involved in transmitting external growth signals to the cell's interior leading to varied biological responses. They interact with other pathway components including GRB2 and SOS which further propagate the signal cascade. This places FRS2 and FRS3 at a central point in controlling cellular responses to external stimuli.

Mutations or malfunctions in FRS2 and FRS3 have been implicated in several conditions notably cancer and developmental disorders. Aberrant signaling through these adaptors can lead to uncontrolled cell proliferation contributing to oncogenesis. Additionally they have connections to developmental defects where improper signaling affects organogenesis and tissue formation. These proteins interact with key components such as FGFRs which are known to be altered in these disease states further linking FRS2 and FRS3 to the pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
See full target information FRS2

Additional targets

Fibroblast growth factor receptor substrate 3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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