AB273582

Anti-FRZB antibody [EPR23904-20]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal FRZB antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Fiz, Fre, Frzb1, Sfrp3, Frzb, Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1

4 Images

Flow Cytometry (Intracellular) - Anti-FRZB antibody [EPR23904-20] (AB273582)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FRZB antibody [EPR23904-20] (AB273582)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling FRZB with ab273582 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (AB273582)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (AB273582)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling FRZB with ab273582 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Unknown

Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (AB273582)

FRZB was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab273582 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273582 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10 ug

Lane 2 : ab273582 IP in Mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273582 in mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (ab273582)

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Lab

Western blot - Anti-FRZB antibody [EPR23904-20] (AB273582)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28300015).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Carrier free

Anti-FRZB antibody [EPR23904-20] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23904-20

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRZB also known as secreted frizzled-related protein 3 (SFRP3) functions as a modulator of Wnt signaling pathways. This protein has a mass of approximately 36 kDa and serves as an antagonist to the Wnt proteins by binding to them preventing their interaction with cell surface receptors. FRZB is expressed in several tissues with notable levels in cartilage bone and the heart.

Biological function summary

The modulation of Wnt signaling by FRZB plays a significant role in embryonic development and tissue homeostasis. FRZB is not part of a protein complex but has important interactions with other components of the signaling cascade. It influences cell differentiation proliferation and apoptosis which are critical processes during development and tissue repair.

Pathways

FRZB influences both Wnt/β-catenin and non-canonical Wnt pathways. Its interaction with these pathways regulates signal transmission that affects cellular behaviors. Related proteins include Wnt ligands and frizzled receptors which are central to these signaling mechanisms.

Abnormalities in FRZB expression or function show a connection to osteoarthritis and certain cancers. In osteoarthritis FRZB interacts with Wnt proteins which contribute to the degradation of cartilage. In cancer dysregulation of Wnt signaling involving FRZB can lead to unchecked cell proliferation. Understanding its role in these diseases can aid in developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development (By similarity).

See full target information Frzb

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40257651

2025

PTGS2 Silencing Inhibits Ferroptosis in Staphylococcus Aureus-induced Osteomyelitis By Blocking the IL-17A Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Rui Zhou,Wen-Guang Li,Li-Dan Yang,Hao Xiang,Ying Jin,Jian-Bo Feng,Hua-Zhang Xiong,Jiachen Peng

Inflammation and regeneration 44:3 PubMed38191481

2024

Th22 is the effector cell of thymosin β15-induced hair regeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nana Tao,Yuyuan Ying,Xie Xu,Qingru Sun,Yaoying Shu,Shiyu Hu,Zhaohuan Lou,Jianli Gao

Experimental and therapeutic medicine 26:531 PubMed37869648

2023

FRZB affects aureus‑induced osteomyelitis in human bone marrow derived stem cells by regulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Wenyong Pang,Hongsong Fan,Hao Wang,Leibing Zhang

Aging 13:11281-11295 PubMed33820867

2021

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Xu,Fei Zhang,Junlan Lu,Kexin Wang,Peng Pan,Ye Sun,Yuxin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FRZB antibody [EPR23904-20]

Flow Cytometry (Intracellular) - Anti-FRZB antibody [EPR23904-20] (AB273582)

Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (AB273582)

Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (AB273582)

Western blot - Anti-FRZB antibody [EPR23904-20] (AB273582)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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