Anti-FRZB antibody [EPR29379-32] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-FRZB antibody [EPR29379-32] - BSA and Azide free (AB324820)

This data was developed using ab322108, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FRZB antibody [EPR29379-32] (ab322108) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

IMR-32 transfected with siRNA specifically targeting FRZB whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-FRZB antibody [EPR29379-32] - BSA and Azide free (AB324820)

This data was developed using ab322108, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : human liver and lung.

Lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FRZB antibody [EPR29379-32] (ab322108) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution

Observed band size: 36 kDa

false

Exposure time: 92s