AB180489

Anti-FSH beta antibody [EPR7931]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal FSH beta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Follitropin subunit beta, Follicle-stimulating hormone beta subunit, Follitropin beta chain, FSH-B, FSH-beta, FSHB

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-FSH beta antibody [EPR7931] (AB180489)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FSH beta antibody [EPR7931] (AB180489)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling FSH beta with ab180489 at 1/100 dilution followed by Goat anti rabbit IgG (Dylight 488) secondary antibody at 1/250 dilution. Counter stained with Dapi.

Supplier Data

Western blot - Anti-FSH beta antibody [EPR7931] (AB180489)

All lanes:

Western blot - Anti-FSH beta antibody [EPR7931] (ab180489) at 1/1000 dilution

All lanes:

Human pituitary lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 15 kDa

false

Carrier free

Anti-FSH beta antibody [EPR7931] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7931

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FSH beta subunit known as hFSH or beta FSH is a part of the follicle-stimulating hormone (FSH) which plays a critical role in reproductive processes. The FSH protein consists of two subunits alpha and beta where the beta subunit is responsible for its biological specificity and binding activity. The genes encoding these subunits are mapped on different chromosomes. The FSH beta gene is expressed mainly in the pituitary gland and produces a protein with a molecular mass around 22 kDa. Its expression can vary depending on the physiological needs of the body such as during different phases of the menstrual cycle.

Biological function summary

The hormone FSH stimulates ovarian follicle growth in females and spermatogenesis in males. It acts as part of a larger heterodimeric complex with the alpha subunit. The interactions of these subunits are essential for the function of FSH enabling it to bind to specific cell surface receptors present on target tissues like the gonads. This binding triggers intracellular signaling cascades that facilitate reproductive maturation and function. The intricate behavior of FSH highlights its role in fertility and reproductive health.

Pathways

FSH beta subunit contributes critically to the reproductive hormone signaling and MAPK pathways. It interacts with other reproductive hormones such as luteinizing hormone (LH) to regulate reproductive processes. Upon binding to its receptor FSH activates pathways involving cyclic adenosine monophosphate (cAMP) that mediate ovarian and testicular function. These interactions highlight the role of FSH in coordinating with other proteins to maintain reproductive health and hormonal balance.

FSH plays an important role in conditions like polycystic ovary syndrome (PCOS) and hypogonadism. Altered levels of FSH can lead to abnormal reproductive functions infertility and irregular menstrual cycles. Its interaction with proteins such as inhibin and activin affects its secretion and action. Therapies targeting FSH levels or its signaling pathways can be pivotal in managing these reproductive disorders highlighting its clinical significance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways (PubMed : 24692546, PubMed : 2494176). Follitropin is involved in follicle development and spermatogenesis in reproductive organs (PubMed : 407105, PubMed : 8220432).

See full target information FSHB

Additional targets

FSH beta

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Endocrine 83:733-746 PubMed37966704

2023

Changes in pituitary gonadotropin subunits and hypothalamic Kiss-1 gene expression by administration of sex steroids in ovary-intact female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Susdiaman S Yacca,Haruhiko Kanasaki,Tuvshintugs Tumurbaatar,Zhuoma Cairang,Aki Oride,Hiroe Okada,Satoru Kyo

International journal of endocrinology 2023:3143347 PubMed36941851

2023

Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Aki Oride,Haruhiko Kanasaki,Tuvshintugs Tumurbaatar,Zolzaya Tumurgan,Hiroe Okada,Zhuoma Cairang,Kyo Satoru

Cells 11: PubMed35326436

2022

The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wan,Hua Yang,Peiyong Chen,Zhibo Wang,Yu Cai,Xiaolei Yao,Feng Wang,Yanli Zhang

Nature communications 11:5275 PubMed33077725

2020

Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Zhang,Yueli Cui,Xinyi Ma,Jun Yong,Liying Yan,Ming Yang,Jie Ren,Fuchou Tang,Lu Wen,Jie Qiao

PloS one 12:e0174626 PubMed28362807

2017

Role of keratin 24 in human epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Min Min,Xi-Bei Chen,Ping Wang,Lilla Landeck,Jia-Qi Chen,Wei Li,Sui-Qing Cai,Min Zheng,Xiao-Yong Man

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FSH beta antibody [EPR7931]

Immunocytochemistry/ Immunofluorescence - Anti-FSH beta antibody [EPR7931] (AB180489)

Western blot - Anti-FSH beta antibody [EPR7931] (AB180489)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

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