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AB250192

Anti-FSH beta antibody [EPR7931] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FSH beta antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Follitropin subunit beta, Follicle-stimulating hormone beta subunit, Follitropin beta chain, FSH-B, FSH-beta, FSHB

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FSH beta antibody [EPR7931] - BSA and Azide free (AB250192)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FSH beta antibody [EPR7931] - BSA and Azide free (AB250192)

This data was developed using ab180489, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling FSH beta with ab180489 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) secondary antibody at 1/250 dilution. Counter stained with Dapi.

Western blot - Anti-FSH beta antibody [EPR7931] - BSA and Azide free (AB250192)
  • WB

Supplier Data

Western blot - Anti-FSH beta antibody [EPR7931] - BSA and Azide free (AB250192)

This data was developed using ab180489, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FSH beta antibody [EPR7931] (<a href='/en-us/products/primary-antibodies/fsh-beta-antibody-epr7931-ab180489'>ab180489</a>) at 1/1000 dilution

All lanes:

Human pituitary lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7931

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250192 is the carrier-free version of ab180489.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FSH beta subunit known as hFSH or beta FSH is a part of the follicle-stimulating hormone (FSH) which plays a critical role in reproductive processes. The FSH protein consists of two subunits alpha and beta where the beta subunit is responsible for its biological specificity and binding activity. The genes encoding these subunits are mapped on different chromosomes. The FSH beta gene is expressed mainly in the pituitary gland and produces a protein with a molecular mass around 22 kDa. Its expression can vary depending on the physiological needs of the body such as during different phases of the menstrual cycle.
Biological function summary

The hormone FSH stimulates ovarian follicle growth in females and spermatogenesis in males. It acts as part of a larger heterodimeric complex with the alpha subunit. The interactions of these subunits are essential for the function of FSH enabling it to bind to specific cell surface receptors present on target tissues like the gonads. This binding triggers intracellular signaling cascades that facilitate reproductive maturation and function. The intricate behavior of FSH highlights its role in fertility and reproductive health.

Pathways

FSH beta subunit contributes critically to the reproductive hormone signaling and MAPK pathways. It interacts with other reproductive hormones such as luteinizing hormone (LH) to regulate reproductive processes. Upon binding to its receptor FSH activates pathways involving cyclic adenosine monophosphate (cAMP) that mediate ovarian and testicular function. These interactions highlight the role of FSH in coordinating with other proteins to maintain reproductive health and hormonal balance.

FSH plays an important role in conditions like polycystic ovary syndrome (PCOS) and hypogonadism. Altered levels of FSH can lead to abnormal reproductive functions infertility and irregular menstrual cycles. Its interaction with proteins such as inhibin and activin affects its secretion and action. Therapies targeting FSH levels or its signaling pathways can be pivotal in managing these reproductive disorders highlighting its clinical significance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways (PubMed : 24692546, PubMed : 2494176). Follitropin is involved in follicle development and spermatogenesis in reproductive organs (PubMed : 407105, PubMed : 8220432).
See full target information FSHB

Additional targets

FSH beta
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

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