Anti-FSHR antibody [HL2784] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325857 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (ab325857) at 1/1000 dilution

All lanes:

Human ovary tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325857 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (ab325857) at 1/1000 dilution

Lane 1:

Boiled mouse ovary tissue extracts at 50 µg

Lane 2:

Boiled mouse muscle tissue extracts at 50 µg

Lane 3:

Unboiled mouse ovary tissue extracts at 50 µg

Lane 4:

Unboiled mouse muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

ab325857 detects FSH Receptor protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : FSH Receptor stained by ab325857 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325857 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (ab325857) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged FSHR transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged FSHR transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false