Rabbit Polyclonal FSP27 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CIDEC aa 1 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CIDEC aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96AQ7
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.
Alternative names
Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog, FSP27, CIDEC
Recommended products
Rabbit Polyclonal FSP27 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CIDEC aa 1 to C-terminus.
Alternative names
Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog, FSP27, CIDEC
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CIDEC aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96AQ7
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
FSP27 also known as CIDEC (cell death-inducing DFFA-like effector C) is a protein involved in lipid droplet formation and is composed of about 238 amino acids with a mass of approximately 27 kDa. Researchers observe its expression predominantly in adipose tissue. As an important component FSP27 contributes to the regulation and accumulation of lipids within cells particularly in white adipose tissue where it facilitates triglyceride storage.
- Biological function summary
FSP27 plays a critical role in lipid metabolism and energy homeostasis. It localizes on the surface of lipid droplets where it interacts with other structural proteins to maintain the stability and size of these lipid storage structures within adipocytes. While it does not form a traditional protein complex its function integrates with the overall architecture of lipid droplets to ensure efficient fat storage and energy balance in the body.
- Pathways
FSP27 significantly impacts the regulation of lipid metabolism and energy homeostasis pathways. It associates with the perilipin family of proteins which also manage lipid droplet dynamics important to adipocyte function. The protein interacts with pathways like the insulin signaling pathway and the PPARγ signaling pathway important for maintaining metabolic homeostasis and regulating adipocyte differentiation. These interactions ensure proper energy storage and expenditure balance playing a vital role in metabolic health.
- Associated diseases and disorders
FSP27 has strong associations with obesity and type 2 diabetes. This protein's function in lipid storage and energy regulation links it with metabolic syndrome development. When dysregulated it may contribute to excess adipose tissue growth leading to obesity and impaired metabolic pathways advancing insulin resistance seen in type 2 diabetes. It interacts notably with proteins such as adiponectin which is known to influence glucose regulation and fatty acid breakdown further connecting FSP27 to metabolic disruptions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-FSP27 antibody (ab102016)
12% SDS PAGE
All lanes: Western blot - Anti-FSP27 antibody (ab102016) at 1/1000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 27 kDa
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