AB198204

Anti-FSP27 antibody

Name: Anti-FSP27 antibody (ab198204) | Abcam
Brand: Abcam Limited
SKU: AB198204
Price: 420 USD
Availability: InStock

(1 Review)

(9 Publications)

Rabbit Polyclonal FSP27 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CIDEC.

View Alternative Names

FSP27, CIDEC, Lipid transferase CIDEC, Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB198204)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB198204)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling FSP27 with ab198204 at a 1/50 dilution.

Supplier Data

Western blot - Anti-FSP27 antibody (AB198204)

8% SDS-PAGE

All lanes:

Western blot - Anti-FSP27 antibody (ab198204) at 1/551 dilution

Lane 1:

Mouse intestinum tenue tissue lysate at 40 µg

Lane 2:

Mouse heart tissue lysate at 40 µg

Predicted band size: 27 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CIDEC.

Q96AQ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSP27 also known as CIDEC (cell death-inducing DFFA-like effector C) is a protein involved in lipid droplet formation and is composed of about 238 amino acids with a mass of approximately 27 kDa. Researchers observe its expression predominantly in adipose tissue. As an important component FSP27 contributes to the regulation and accumulation of lipids within cells particularly in white adipose tissue where it facilitates triglyceride storage.

Biological function summary

FSP27 plays a critical role in lipid metabolism and energy homeostasis. It localizes on the surface of lipid droplets where it interacts with other structural proteins to maintain the stability and size of these lipid storage structures within adipocytes. While it does not form a traditional protein complex its function integrates with the overall architecture of lipid droplets to ensure efficient fat storage and energy balance in the body.

Pathways

FSP27 significantly impacts the regulation of lipid metabolism and energy homeostasis pathways. It associates with the perilipin family of proteins which also manage lipid droplet dynamics important to adipocyte function. The protein interacts with pathways like the insulin signaling pathway and the PPARγ signaling pathway important for maintaining metabolic homeostasis and regulating adipocyte differentiation. These interactions ensure proper energy storage and expenditure balance playing a vital role in metabolic health.

FSP27 has strong associations with obesity and type 2 diabetes. This protein's function in lipid storage and energy regulation links it with metabolic syndrome development. When dysregulated it may contribute to excess adipose tissue growth leading to obesity and impaired metabolic pathways advancing insulin resistance seen in type 2 diabetes. It interacts notably with proteins such as adiponectin which is known to influence glucose regulation and fatty acid breakdown further connecting FSP27 to metabolic disruptions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566, PubMed : 30361435). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1 (PubMed : 23399566). May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (By similarity). When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment (PubMed : 12429024).

See full target information CIDEC

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102347 PubMed35963433

2022

Human CIDEC transgene improves lipid metabolism and protects against high-fat diet-induced glucose intolerance in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Gupta,Bijinu Balakrishnan,Shakun Karki,Mark Slayton,Sukanta Jash,Sayani Banerjee,Tan Hooi Min Grahn,Srikarthika Jambunathan,Sarah Disney,Hebaallaha Hussein,Dong Kong,Bradford B Lowell,Purushothaman Natarajan,Umesh K Reddy,Noyan Gokce,Vishva M Sharma,Vishwajeet Puri

Journal of dairy science 105:6997-7010 PubMed35688731

2022

Activated autophagy-lysosomal pathway in dairy cows with hyperketonemia is associated with lipolysis of adipose tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yu,Minghe Fan,Xiying Chen,Xiuhuan Jiang,Juan J Loor,Ahmad Aboragah,Cai Zhang,Hongxu Bai,Zhiyuan Fang,Taiyu Shen,Zhe Wang,Yuxiang Song,Xinwei Li,Guowen Liu,Xiaobing Li,Xiliang Du

Current issues in molecular biology 43:1828-1843 PubMed34889901

2021

Losartan Attenuates Insulin Resistance and Regulates Browning Phenomenon of White Adipose Tissue in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hsuan-Miao Liu,Cheng-Hui Wang,Zi-Yu Chang,Tse-Hung Huang,Tzung-Yan Lee

International journal of molecular sciences 22: PubMed34360607

2021

Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1α in a Murine Model of NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Hui Wang,Hsuan-Miao Liu,Zi-Yu Chang,Tse-Hung Huang,Tzung-Yan Lee

Journal of dairy science 104:6134-6145 PubMed33685683

2021

Inhibition of cell death inducing DNA fragmentation factor-α-like effector c (CIDEC) by tumor necrosis factor-α induces lipolysis and inflammation in calf adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Minghe Fan,Xiliang Du,Xiying Chen,Hongxu Bai,Juan J Loor,Taiyu Shen,Yusheng Liang,Xudong Sun,Qiushi Xu,Yuxiang Song,Zhe Wang,Guowen Liu,Liangyu Yang,Xinwei Li,Xiaobing Li,Wenwen Gao

Journal of dairy science 104:6212-6221 PubMed33663853

2021

Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) regulates acetate- and β-hydroxybutyrate-induced milk fat synthesis by increasing FASN expression in mammary epithelial cells of dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

He Lv,Qingyu Meng,Nan Wang,Xiaoyu Duan,Xiaoming Hou,Ye Lin

Scientific reports 9:7501 PubMed31097771

2019

The Differential Expression of Cide Family Members is Associated with Nafld Progression from Steatosis to Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Arnaud Sans,Stéphanie Bonnafous,Déborah Rousseau,Stéphanie Patouraux,Clémence M Canivet,Pierre S Leclere,Jeanne Tran-Van-Nhieu,Carmelo Luci,Béatrice Bailly-Maitre,Xu Xu,Ann-Hwee Lee,Kaori Minehira,Rodolphe Anty,Albert Tran,Antonio Iannelli,Philippe Gual

BioMed research international 2019:6415732 PubMed31205943

2019

Bariatric Surgery in Rats Upregulates FSP27 Expression in Fat Tissue to Affect Fat Hydrolysis and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyao Hu,Mofei Wang,Yong Zhou,Xiaowei Zhang,Bing He,Lei Liu,Rui Ma,Tianyi Zhang,Keyi Liu,Yong Wang,Jingang Liu

Journal of dairy science 100:4014-4024 PubMed28284693

2017

The effects of cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) on milk lipid synthesis in mammary glands of dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Ye Lin,Xiaoyu Duan,He Lv,Weinan Xing,Qingzhang Li,Xuejun Gao,Xiaoming Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FSP27 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB198204)

Western blot - Anti-FSP27 antibody (AB198204)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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