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AB213693

Anti-FSP27 antibody

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(3 Publications )

Rabbit Polyclonal FSP27 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CIDEC aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FSP27, CIDEC, Lipid transferase CIDEC, Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling FSP27 with ab213693 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse stomach tissue labeling FSP27 with ab213693 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSP27 antibody (AB213693)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat heart tissue labeling FSP27 with ab213693 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Western blot - Anti-FSP27 antibody (AB213693)
  • WB

Lab

Western blot - Anti-FSP27 antibody (AB213693)

All lanes:

Western blot - Anti-FSP27 antibody (ab213693) at 1/500 dilution

All lanes:

Mouse epididymis lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CIDEC aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96AQ7

Specificity

Due to the similarity of this protein with CIDEA in Mouse and Rat, there is a chance that this antibody will react with this protein in those two species based on homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSP27 also known as CIDEC (cell death-inducing DFFA-like effector C) is a protein involved in lipid droplet formation and is composed of about 238 amino acids with a mass of approximately 27 kDa. Researchers observe its expression predominantly in adipose tissue. As an important component FSP27 contributes to the regulation and accumulation of lipids within cells particularly in white adipose tissue where it facilitates triglyceride storage.
Biological function summary

FSP27 plays a critical role in lipid metabolism and energy homeostasis. It localizes on the surface of lipid droplets where it interacts with other structural proteins to maintain the stability and size of these lipid storage structures within adipocytes. While it does not form a traditional protein complex its function integrates with the overall architecture of lipid droplets to ensure efficient fat storage and energy balance in the body.

Pathways

FSP27 significantly impacts the regulation of lipid metabolism and energy homeostasis pathways. It associates with the perilipin family of proteins which also manage lipid droplet dynamics important to adipocyte function. The protein interacts with pathways like the insulin signaling pathway and the PPARγ signaling pathway important for maintaining metabolic homeostasis and regulating adipocyte differentiation. These interactions ensure proper energy storage and expenditure balance playing a vital role in metabolic health.

FSP27 has strong associations with obesity and type 2 diabetes. This protein's function in lipid storage and energy regulation links it with metabolic syndrome development. When dysregulated it may contribute to excess adipose tissue growth leading to obesity and impaired metabolic pathways advancing insulin resistance seen in type 2 diabetes. It interacts notably with proteins such as adiponectin which is known to influence glucose regulation and fatty acid breakdown further connecting FSP27 to metabolic disruptions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566, PubMed : 30361435). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (PubMed : 18334488, PubMed : 19843876, PubMed : 20049731, PubMed : 23399566). Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1 (PubMed : 23399566). May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (By similarity). When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment (PubMed : 12429024).
See full target information CIDEC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 11:6686-6697 PubMed37823117

2023

Effect of vitamin D3 on lipid droplet growth in adipocytes of mice with HFD-induced obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Yuanfan Zhang,Yong Zhou,Wenxin Zhao,Jialu Li,Dan Yang,Lian Xiang,Tingwan Du,Ling Ma

International journal of molecular sciences 23: PubMed35682666

2022

Metformin Inhibits Lipid Droplets Fusion and Growth via Reduction in Cidec and Its Regulatory Factors in Rat Adipose-Derived Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lijing Yang,Xiaowei Jia,Dongliang Fang,Yuan Cheng,Zhaoyi Zhai,Wenyang Deng,Baopu Du,Tao Lu,Lulu Wang,Chun Yang,Yan Gao

Molecular medicine reports 16:1871-1877 PubMed28656280

2017

Role of glycoprotein 78 and cidec in hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Guocai Liu,Feng Zhang,Zhiwen Zhang,Yuqiao Xu,Qing Li
View all publications
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