Anti-FSP27/CIDEC antibody [EPR29812-32]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FSP27/CIDEC antibody [EPR29812-32] (AB325929)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) and Differentiated 3T3-L1 cells labelling FSP27 with ab325929 at 1/250 (2.084 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in Differentiated 3T3-L1 cells and no staining in 3T3-L1 cells(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

• WB

Lab

Western blot - Anti-FSP27/CIDEC antibody [EPR29812-32] (AB325929)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, kidney (PMID : 18654663; PMID : 18682832)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18654663; PMID : 18682832).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FSP27/CIDEC antibody [EPR29812-32] (ab325929) at 1/1000 dilution

Lane 1:

Mouse brown fat tissue lysate at 20 µg

Lane 2:

Mouse white fat tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Rat brown fat tissue lysate at 20 µg

Lane 6:

Rat white fat tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-FSP27/CIDEC antibody [EPR29812-32] (AB325929)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18654663; PMID : 18682832).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-FSP27/CIDEC antibody [EPR29812-32] (ab325929) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

3T3-L1 cells differentiate for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa

false

Exposure time: 70s