AB11805

Anti-FSTL1/FRP antibody

Name: Anti-FSTL1/FRP antibody (ab11805) | Abcam
Brand: Abcam Limited
SKU: AB11805
Rating: 3 (1 reviews)

(1 Review)

(11 Publications)

Goat Polyclonal FSTL1/FRP antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human FSTL1 aa 250 to C-terminus.

View Alternative Names

FRP, FSTL1, Follistatin-related protein 1, Follistatin-like protein 1

1 Images

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Western blot - Anti-FSTL1/FRP antibody (AB11805)

Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-FSTL1/FRP antibody (ab11805) at 0.5 µg/mL

All lanes:

Human Placenta lysate (RIPA buffer, 35µg total protein per lane)

Predicted band size: 35 kDa

Observed band size: 50 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human FSTL1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12841

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.

Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed : 22265692, PubMed : 29212066). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed : 20054002, PubMed : 22265692).

See full target information FSTL1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 10:e15300 PubMed35585770

2022

Aerobic exercise training-induced follistatin-like 1 secretion in the skeletal muscle is related to arterial stiffness via arterial NO production in obese rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Inoue,Shumpei Fujie,Naoki Horii,Henry Yamazaki,Masataka Uchida,Motoyuki Iemitsu

American journal of cancer research 11:4981-4993 PubMed34765305

2021

HuR enhances FSTL1 transcript stability to promote invasion and metastasis of squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gopinath M Sundaram,Shan Quah,Lum Guo Guang,Prabha Sampath

Aging 13:11455-11469 PubMed33839699

2021

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Cao,Xinsheng Han,Yonglin Jia,Baohua Zhang

Genome biology 22:54 PubMed33514403

2021

A comprehensive enhancer screen identifies TRAM2 as a key and novel mediator of YAP oncogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Alejandro P Ugalde,Colinda L G J Scheele,Sebastian M Dieter,Remco Nagel,Jin Ma,Abhijeet Pataskar,Gozde Korkmaz,Ran Elkon,Miao-Ping Chien,Li You,Pin-Rui Su,Onno B Bleijerveld,Maarten Altelaar,Lyubomir Momchev,Zohar Manber,Ruiqi Han,Pieter C van Breugel,Rui Lopes,Peter Ten Dijke,Jacco van Rheenen,Reuven Agami

Oxidative medicine and cellular longevity 2020:3598715 PubMed32831995

2020

Follistatin-Like 1 Protects against Doxorubicin-Induced Cardiomyopathy through Upregulation of Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Yintao Zhao,Jingjing Sun,Wei Zhang,Meng Peng,Jun Chen,Lu Zheng,Xiangqin Zhang,Haibo Yang,Yuan Liu

The Journal of experimental medicine 214:2889-2900 PubMed28827448

2017

EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gopinath M Sundaram,Hisyam M Ismail,Mohsin Bashir,Manish Muhuri,Candida Vaz,Srikanth Nama,Ghim Siong Ow,Ivshina Anna Vladimirovna,Rajkumar Ramalingam,Brian Burke,Vivek Tanavde,Vladimir Kuznetsov,E Birgitte Lane,Prabha Sampath

Placenta 36:1231-8 PubMed26386648

2015

The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Francois Mouillet,Takuya Mishima,Andrea Mollica do Amarante Paffaro,Tony W Parks,Judy A Ziegler,Tianjiao Chu,Yoel Sadovsky

Nature 495:103-6 PubMed23395958

2013

'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Gopinath M Sundaram,John E A Common,Felicia E Gopal,Satyanarayana Srikanta,Krishnaswamy Lakshman,Declan P Lunny,Thiam C Lim,Vivek Tanavde,E Birgitte Lane,Prabha Sampath

American journal of physiology. Renal physiology 299:F1320-7 PubMed20861081

2010

Follistatin-like 1 regulates renal IL-1β expression in cisplatin nephrotoxicity.

Applications

Species

Human

Derek C Adams,Michele J Karolak,Barry W Larman,Lucy Liaw,James D Nolin,Leif Oxburgh

Journal of proteome research 9:6-21 PubMed19678649

2009

Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.

Applications

Species

Human

Astrid Slany,Verena J Haudek,Hannes Zwickl,Nina C Gundacker,Michael Grusch,Thomas S Weiss,Katja Seir,Chantal Rodgarkia-Dara,Claus Hellerbrand,Christopher Gerner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FSTL1/FRP antibody

Western blot - Anti-FSTL1/FRP antibody (AB11805)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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