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Rabbit Recombinant Monoclonal FSTL1/FRP antibody. Suitable for IP, WB and reacts with Rat, Mouse, Human samples. Cited in 6 publications.

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Images

Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (AB223287), expandable thumbnail
  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (AB223287), expandable thumbnail
  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (AB223287), expandable thumbnail
  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (AB223287), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Expected
Tested
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed:22265692, PubMed:29212066). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed:20054002, PubMed:22265692).

Alternative names

FRP, FSTL1, Follistatin-related protein 1, Follistatin-like protein 1

Recommended products

Rabbit Recombinant Monoclonal FSTL1/FRP antibody. Suitable for IP, WB and reacts with Rat, Mouse, Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21933-155
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.

Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

Associated diseases and disorders

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287), expandable thumbnail

    Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)

    FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
    Lane 1: Rat embryo lysate 10 μg (Input).
    Lane 2: ab223287 IP in rat embryo lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab223287 in rat embryo lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 minute.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    All lanes: Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)

    Predicted band size: 35 kDa

    Observed band size: 43-55 kDa

  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287), expandable thumbnail

    Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    Negative control: Rat liver (PMID: 20861081).

    All lanes: Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution

    Lane 1: Rat E14 embryo lysate at 20 µg

    Lane 2: Mouse E14 embryo lysate at 20 µg

    Lane 3: C6 (Rat glial tumor cell line) cell lysate at 20 µg

    Lane 4: Rat liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 43-45 kDa

    Exposure time: 48s

  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287), expandable thumbnail

    Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times:
    Lane 1 and 2: 3 minutes;
    Lane 3: 15 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 27234440).

    All lanes: Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution

    Lane 1: Human serum at 20 µg

    Lane 2: Mouse serum at 20 µg

    Lane 3: Rat serum at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lane 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40-50 kDa

  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287), expandable thumbnail

    Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)

    FSTL1/FRP Western blot staining using rabbit Anti-FSTL1/FRP antibody

    Western blot: Anti-FSTL1/FRP antibody [EPR21933-155] ab223287 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type HeLa cell lysates with no signal observed at this size in FSTL1 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution

    Lane 1: Wild-type HeLa at 20 µg

    Lane 2: Human FSTL1 knockout HeLa cell line at 20 µg

    Lane 3: A431 at 20 µg

    Lane 4: Ramos at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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