Rabbit Recombinant Monoclonal FSTL1/FRP antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | WB | |
---|---|---|
Human | Expected | Tested |
Mouse | Expected | Tested |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
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Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed:22265692, PubMed:29212066). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (By similarity). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (PubMed:20054002, PubMed:22265692).
FRP, FRP, FSTL1, Follistatin-related protein 1, Follistatin-like protein 1
Rabbit Recombinant Monoclonal FSTL1/FRP antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR21933-155
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab234961 is the carrier-free version of Anti-FSTL1/FRP antibody [EPR21933-155] ab223287.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.
FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.
FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.
FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with Anti-FSTL1/FRP antibody [EPR21933-155] ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-FSTL1/FRP antibody [EPR21933-155] ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rat embryo lysate 10 μg (Input).
Lane 2: Anti-FSTL1/FRP antibody [EPR21933-155] ab223287 IP in rat embryo lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FSTL1/FRP antibody [EPR21933-155] ab223287 in rat embryo lysate (-).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FSTL1/FRP antibody [EPR21933-155] ab223287).
All lanes: Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (Anti-FSTL1/FRP antibody [EPR21933-155] ab223287)
Predicted band size: 35 kDa
Observed band size: 43-55 kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
Negative control: Rat liver (PMID: 20861081).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FSTL1/FRP antibody [EPR21933-155] ab223287).
All lanes: Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961) at 1/1000 dilution
Lane 1: Rat E14 embryo lysate at 20 µg
Lane 2: Mouse E14 embryo lysate at 20 µg
Lane 3: C6 (Rat glial tumor cell line) cell lysate at 20 µg
Lane 4: Rat liver lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 43-50 kDa
Exposure time: 48s
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