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AB259629

Anti-FSTL1/FRP antibody [EPR21933-190] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal FSTL1/FRP antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

View Alternative Names

Frp, Fstl1, Follistatin-related protein 1, Follistatin-like protein 1

1 Images
Sandwich ELISA - Anti-FSTL1/FRP antibody [EPR21933-190] - BSA and Azide free (Capture) (AB259629)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FSTL1/FRP antibody [EPR21933-190] - BSA and Azide free (Capture) (AB259629)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab246546).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21933-190

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab259629 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat FSTL1. The recommended pair for sandwich ELISA is:
Capture: ab259629, Rat FSTL1 Capture Antibody (unconjugated)
Detector: ab259630, Rat FSTL1 Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.
Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (By similarity). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (By similarity). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (By similarity).
See full target information Fstl1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com