JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259560

Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal FSTL3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

FLRG, UNQ674/PRO1308, FSTL3, Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein

1 Images
Sandwich ELISA - Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector) (AB259560)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector) (AB259560)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab245715).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21996-225

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab259560 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human FLRG. The recommended pair for sandwich ELISA is:
Capture: ab259559, Human FLRG Capture Antibody (unconjugated)
Detector: ab259560, Human FLRG Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FSTL3 also known as Follistatin-like 3 has a molecular mass of approximately 26 kDa. Mechanically FSTL3 acts by binding and neutralizing specific members of the TGF-beta superfamily influencing cellular processes like growth differentiation and apoptosis. It is mostly expressed in tissues such as the placenta pancreas and muscle where it plays a role in various developmental and physiological events.
Biological function summary

FSTL3 serves an important function in regulating cellular dynamics by modulating activity of ligands in the TGF-beta signaling pathway. It forms a complex with activins and other growth factors to inhibit their signaling capabilities. This interaction is key in processes like managing fetal development ensuring proper organogenesis and maintaining metabolic activities.

Pathways

Extensive research places FSTL3 within the TGF-beta signaling pathway and the insulin-like growth factor (IGF) system. These pathways are critical for managing cellular growth and metabolism. FSTL3 interacts with proteins such as activins and inhibins which share roles in regulating cell proliferation and differentiation linking FSTL3 directly to these essential cellular pathways.

FSTL3 links to pathologies like type 2 diabetes and muscle wasting. FSTL3 influences insulin signaling therefore its dysregulation can contribute to insulin resistance a hallmark of type 2 diabetes. It also associates with muscle protein wasting through its interaction with activin suggesting a potential therapeutic target in conditions like cachexia.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such as activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
See full target information FSTL3
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB245715

Human FLRG ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB181141

Anti-IGFBP1 antibody [EPR14472(B)]

RabMAb|Recombinant

Western blot - Anti-IGFBP1 antibody [EPR14472(B)] (AB181141)

  • 3
  • 1
Primary Antibodies

AB174843

Anti-NBL1 antibody [EPR12397]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NBL1 antibody [EPR12397] (AB174843)

  • 0
  • 0
Primary Antibodies

AB219560

Anti-IGFBP6 antibody [EPR21809]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IGFBP6 antibody [EPR21809] (AB219560)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com