JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB92821

Anti-FTO antibody [5-2H10]

4

(3 Reviews)

|

(76 Publications)

Mouse Monoclonal FTO antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat samples. Cited in 76 publications. Immunogen corresponding to Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA1752, FTO, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO, m6A(m)-demethylase FTO

3 Images
Flow Cytometry - Anti-FTO antibody [5-2H10] (AB92821)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-FTO antibody [5-2H10] (AB92821)

Overlay histogram showing SH-SY5Y cells stained with ab92821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92821, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-FTO antibody [5-2H10] (AB92821)
  • WB

Lab

Western blot - Anti-FTO antibody [5-2H10] (AB92821)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FTO knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HEK293 whole cell lysate (20 μg)
Lane 4 : MOLT4 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab92821 observed at 58 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab92821 was shown to specifically recognize FTO in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when FTO knockout samples were examined. Wild-type and FTO knockout samples were subjected to SDS-PAGE. ab92821 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FTO antibody [5-2H10] (ab92821)

Predicted band size: 58 kDa

false

Western blot - Anti-FTO antibody [5-2H10] (AB92821)
  • WB

Unknown

Western blot - Anti-FTO antibody [5-2H10] (AB92821)

All lanes:

Western blot - Anti-FTO antibody [5-2H10] (ab92821) at 1/1000 dilution

All lanes:

Rat testes lysate

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5-2H10

Isotype

IgG2a

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9C0B1

style
left-column

Alternative Products

Primary Antibodies

AB207665

Alexa Fluor® 488 Anti-FTO antibody [EPR6894]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FTO antibody [EPR6894] (AB207665)

  • 0
  • 0
Primary Antibodies

AB126605

Anti-FTO antibody [EPR6894]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6894] (AB126605)

  • 4
  • 3
Primary Antibodies

AB124892

Anti-FTO antibody [EPR6895]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] (AB124892)

  • 4
  • 2
Primary Antibodies

AB242390

Anti-FTO antibody [EPR6895] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6895] - BSA and Azide free (AB242390)

  • 0
  • 0
Primary Antibodies

AB240021

Anti-FTO antibody [EPR6894] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTO antibody [EPR6894] - BSA and Azide free (AB240021)

  • 0
  • 0

Complementary Products

Assay Kits

AB309316

Human FTO ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FTO ELISA Kit (AB309316)

  • 0
  • 0
Primary Antibodies

AB195352

Anti-METTL3 antibody [EPR18810]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry - Anti-METTL3 antibody [EPR18810] (AB195352)

  • 5
  • 8
Primary Antibodies

AB195377

Anti-ALKBH5 antibody [EPR18958]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH5 antibody [EPR18958] (AB195377)

  • 4
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Protein G purified culture supernatant.
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FTO protein also referred to as Alpha-Ketoglutarate-Dependent Dioxygenase or Fat Mass and Obesity-Associated protein functions as an enzyme involved in demethylating nucleic acids specifically N6-methyladenosine (m6A) in RNA. This process impacts RNA stability and translation. FTO has a molecular weight of approximately 58 kDa. Higher expression levels of FTO are found in the brain including the hypothalamus which is an area known for regulating energy balance and appetite.
Biological function summary

FTO influences energy homeostasis and adipogenesis. It acts as an important player in metabolic regulation and gene expression by modulating the m6A RNA modification. While FTO operates largely on its own its demethylation activity may interact with other RNA binding proteins that recognize m6A-modified RNAs integrating it into larger regulatory frameworks for RNA metabolism and cellular responses.

Pathways

FTO is an important component in the regulation of energy balance and metabolism. The protein is closely associated with the mTOR signaling pathway which is essential for controlling cell growth proliferation and survival in response to nutrient availability. FTO also interacts with the leptin signaling pathway relating to its role in appetite regulation and energy expenditure.

FTO has significant connections to obesity and Type 2 diabetes. Genetic variants within the FTO gene correlate with risk for these conditions linking its enzymatic activity to alterations in energy balance and insulin sensitivity. FTO's role in Type 2 diabetes also highlights its interaction with insulin signaling proteins demonstrating its broader influence on glucose metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed : 22002720, PubMed : 25452335, PubMed : 26457839, PubMed : 26458103, PubMed : 28002401, PubMed : 30197295). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed : 22002720, PubMed : 25452335, PubMed : 26457839, PubMed : 26458103, PubMed : 30197295). M6A demethylation by FTO affects mRNA expression and stability (PubMed : 30197295). Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (PubMed : 30197295). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed : 28002401, PubMed : 30197295). Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping (PubMed : 28002401). Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs (PubMed : 30197295). Has no activity towards 1-methylguanine (PubMed : 20376003). Has no detectable activity towards double-stranded DNA (PubMed : 20376003). Also able to repair alkylated DNA and RNA by oxidative demethylation : demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed : 18775698, PubMed : 20376003). Ability to repair alkylated DNA and RNA is however unsure in vivo (PubMed : 18775698, PubMed : 20376003). Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation (PubMed : 18775698, PubMed : 20376003). Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed : 26287746). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity). Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation : acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression (PubMed : 28017614, PubMed : 29249359).
See full target information FTO
style
left-column

Publications (76)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:397 PubMed40846737

2025

WTAP participates in the DNA damage response via an mA-FOXM1-dependent manner in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Huang,Zhixuan Bian,Chang Xu,Yue Zhang,Li Liu,Zhongqi Cui,Shasha Zhao,Qiangyuan Fan,Shaobo Xue,Yan Chen,Qiuhui Pan,Fenyong Sun

International journal of molecular sciences 26: PubMed40362608

2025

An Investigation of the RNA Modification mA and Its Regulatory Enzymes in Rat Brains Affected by Chronic Morphine Treatment and Withdrawal.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Hronova,Eliska Pritulova,Lucie Hejnova,Jiri Novotny

International journal of molecular sciences 26: PubMed39940841

2025

The RNA Demethyltransferase FTO Regulates Ferroptosis in Major Depressive Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Meng,Zijing Liu,Yuesong Yu,Erning Zhang,Xiaolin Yu,Peixin Meng,Jianbo Xiu

CytoJournal 21:83 PubMed39917013

2025

FTO-mediated regulation of Kupffer cell polarization and interleukin-6 secretion promotes biliary epithelial cell proliferation in intrahepatic bile duct stones.

Applications

Unspecified application

Species

Unspecified reactive species

Lixiang Li,Hui Peng,Ziyi Li,Fuhai Zhou,Qingsheng Yu

Journal of virology 99:e0201924 PubMed39791911

2025

The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth Verhamme,Robert J J Jansens,Jianheng Liu,Fien Van Raemdonck,Cliff Van Waesberghe,Luke Nicholson,Samie R Jaffrey,Herman W Favoreel

Nature neuroscience 28:280-292 PubMed39762660

2025

Aberrant splicing in Huntington's disease accompanies disrupted TDP-43 activity and altered m6A RNA modification.

Applications

Unspecified application

Species

Unspecified reactive species

Thai B Nguyen,Ricardo Miramontes,Carlos Chillon-Marinas,Roy Maimon,Sonia Vazquez-Sanchez,Alice L Lau,Nicolette R McClure,Zhuoxing Wu,Keona Q Wang,Whitney E England,Monika Singha,Jennifer T Stocksdale,Marie Heath,Ki-Hong Jang,Sunhee Jung,Karen Ling,Paymann Jafar-Nejad,Jharrayne I McKnight,Leanne N Ho,Osama Al Dalahmah,Richard L M Faull,Joan S Steffan,Jack C Reidling,Cholsoon Jang,Gina Lee,Don W Cleveland,Clotilde Lagier-Tourenne,Robert C Spitale,Leslie M Thompson

Frontiers in pharmacology 15:1491546 PubMed39584135

2024

Huyang Yangkun formula regulates the mitochondria pathway of ovarian granulosa cell apoptosis through FTO/m6A-P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Lingdi Wang,Jian Liu,Guangning Nie,Hongyan Yang

Proceedings of the National Academy of Sciences of the United States of America 121:e2317847121 PubMed39495907

2024

Dynamic multilayered control of mA RNA demethylase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Carine Jaafar,Ricardo C T Aguiar

Molecular therapy : the journal of the American Society of Gene Therapy 32:4418-4434 PubMed39473181

2024

Host RNA N-methyladenosine and incoming DNA N-methyldeoxyadenosine modifications cooperatively elevate the condensation potential of DNA to activate immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wang,Qiaoling Liu,Bo Wang,Zhuo Yang,Siru Li,Ran Li,Xinyuan Liang,Jiayu Fan,Hui Wang,Zhen Sun,Ling Dong,Yueru Hou,Shengnan Wang,Chengli Song,Yang Wang,Chunshan Quan,Qingkai Yang,Lina Wang

Molecular pain 20:17448069241295987 PubMed39415414

2024

Fat mass and obesity-related protein contributes to the development and maintenance of bone cancer pain in rats by abrogating m6A methylation of RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Liu,Danyang Meng,Man Luo,Longsheng Xu,Ming Yao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com