Mouse Monoclonal FTO antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin.
Images
![Flow Cytometry - Anti-FTO antibody [5-2H10] (AB92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--flow-cytometry-img71583.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-FTO antibody [5-2H10] (AB92821)")
![Western blot - Anti-FTO antibody [5-2H10] (AB92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--western-blot-img143292.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FTO antibody [5-2H10] (AB92821)")
![Western blot - Anti-FTO antibody [5-2H10] (AB92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--western-blot-img4788.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FTO antibody [5-2H10] (AB92821)")
Publications
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- Brain circulation 9:162-1712023Glycogen synthase kinase-3β mediates toll-like receptors 4/nuclear factor kappa-B-activated cerebral ischemia-reperfusion injury through regulation of fat mass and obesity-associated protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiwei Xu,Wenwen Du,Xiuxiu Zhuang,Dongdong Liang,Yunchang Mo,Junlu WangPubMed 38020949
- Cell death & disease 14:3852023High glucose induces tau hyperphosphorylation in hippocampal neurons via inhibition of ALKBH5-mediated Dgkh mA demethylation: a potential mechanism for diabetic cognitive dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinli Qu,Linhui Zuo,Mengru Zhang,Peng Cheng,Zhanjun Guo,Junya Yang,Changjun Li,Jing WuPubMed 37385994
- Journal of neurochemistry 166:328-3452023Astragaloside IV alleviates neuronal ferroptosis in ischemic stroke by regulating fat mass and obesity-associated-N6-methyladenosine-acyl-CoA synthetase long-chain family member 4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenglong Jin,Wenying Gao,Fu Guo,Shaojun Liao,Mingzhe Hu,Tao Yu,Shangzhen Yu,Qing ShiPubMed 37300304
- Nature communications 14:11852023N-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyu Wang,Yan Ding,Ran Li,Rujun Zhang,Xuejun Ge,Ruifang Gao,Miao Wang,Yubing Huang,Fang Zhang,Bin Zhao,Wang Liao,Jie DuPubMed 36864027
- Physiological research 71:877-8822022Myocardial m6A regulators in postnatal development: effect of sex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Semenovykh,D Benak,K Holzerova,B Cerna,P Telensky,T Vavrikova,F Kolar,J Neckar,M HlavackovaPubMed 36426889
- Journal of virology 96:e00997222022WTAP Targets the METTL3 mA-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew T Sacco,Katherine M Bland,Stacy M HornerPubMed 36314819
- Biomedicines 10:2022Obesity-Related Genes Expression in Testes and Sperm Parameters Respond to GLP-1 and Caloric Restriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna S Correia,Sara C Pereira,Tiago Morais,Ana D Martins,Mariana P Monteiro,Marco G Alves,Pedro F OliveiraPubMed 36289871
- Frontiers in immunology 13:9987562022Epitranscriptome profiling of spleen mRNA mA methylation reveals pathways of host responses to malaria parasite infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuoluo Wang,Jian Wu,Runzhou Liu,Wenjun Chen,Zhichang Pang,Fan Zhou,Lu Xia,Jia Huang,Tao Pan,Xin-Zhuan Su,Xiaoyun WangPubMed 36203583
- Frontiers in cell and developmental biology 10:9547692022Exercise training ameliorates myocardial phenotypes in heart failure with preserved ejection fraction by changing N6-methyladenosine modification in mice model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Liu,Wenhao Ju,Shengrong Ouyang,Zhuo Liu,Feng He,Jingyi Hao,Hongyan Guan,Jianxin WuPubMed 36120562
- International journal of biological sciences 18:4118-41342022Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Li,Xin Yi,Yi He,Bo Huo,Yue Chen,Zihao Zhang,Qunhui Wang,Yi Li,Xiaoxuan Zhong,Rui Li,Xue-Hai Zhu,Zemin Fang,Xiang Wei,Ding-Sheng JiangPubMed 35844806
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0B1
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Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Rat | Expected | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Primates | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Cow, Dog, Primates | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Cow, Dog, Primates | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Cow, Dog, Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:22002720, PubMed:25452335, PubMed:26457839, PubMed:26458103, PubMed:28002401, PubMed:30197295). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:25452335, PubMed:26457839, PubMed:26458103, PubMed:30197295). M6A demethylation by FTO affects mRNA expression and stability (PubMed:30197295). Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (PubMed:30197295). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed:28002401, PubMed:30197295). Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping (PubMed:28002401). Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs (PubMed:30197295). Has no activity towards 1-methylguanine (PubMed:20376003). Has no detectable activity towards double-stranded DNA (PubMed:20376003). Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Ability to repair alkylated DNA and RNA is however unsure in vivo (PubMed:18775698, PubMed:20376003). Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity). Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression (PubMed:28017614, PubMed:29249359).
Alternative names
KIAA1752, FTO, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO, m6A(m)-demethylase FTO
Recommended products
Mouse Monoclonal FTO antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat samples. Cited in 51 publications. Immunogen corresponding to Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human FTO conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0B1
- Clone number
- 5-2H10
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Protein G purified culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FTO protein also referred to as Alpha-Ketoglutarate-Dependent Dioxygenase or Fat Mass and Obesity-Associated protein functions as an enzyme involved in demethylating nucleic acids specifically N6-methyladenosine (m6A) in RNA. This process impacts RNA stability and translation. FTO has a molecular weight of approximately 58 kDa. Higher expression levels of FTO are found in the brain including the hypothalamus which is an area known for regulating energy balance and appetite.
- Biological function summary
FTO influences energy homeostasis and adipogenesis. It acts as an important player in metabolic regulation and gene expression by modulating the m6A RNA modification. While FTO operates largely on its own its demethylation activity may interact with other RNA binding proteins that recognize m6A-modified RNAs integrating it into larger regulatory frameworks for RNA metabolism and cellular responses.
- Pathways
FTO is an important component in the regulation of energy balance and metabolism. The protein is closely associated with the mTOR signaling pathway which is essential for controlling cell growth proliferation and survival in response to nutrient availability. FTO also interacts with the leptin signaling pathway relating to its role in appetite regulation and energy expenditure.
- Associated diseases and disorders
FTO has significant connections to obesity and Type 2 diabetes. Genetic variants within the FTO gene correlate with risk for these conditions linking its enzymatic activity to alterations in energy balance and insulin sensitivity. FTO's role in Type 2 diabetes also highlights its interaction with insulin signaling proteins demonstrating its broader influence on glucose metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Flow Cytometry - Anti-FTO antibody [5-2H10] (ab92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--flow-cytometry-img71583.jpg "Flow Cytometry - Anti-FTO antibody [5-2H10] (ab92821)")
Flow Cytometry - Anti-FTO antibody [5-2H10] (ab92821)
Overlay histogram showing SH-SY5Y cells stained with ab92821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92821, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-FTO antibody [5-2H10] (ab92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--western-blot-img143292.jpg "Western blot - Anti-FTO antibody [5-2H10] (ab92821)")
Western blot - Anti-FTO antibody [5-2H10] (ab92821)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: FTO knockout HAP1 whole cell lysate (20 μg)
Lane 3: HEK293 whole cell lysate (20 μg)
Lane 4: MOLT4 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab92821 observed at 58 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab92821 was shown to specifically recognize FTO in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when FTO knockout samples were examined. Wild-type and FTO knockout samples were subjected to SDS-PAGE. ab92821 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FTO antibody [5-2H10] (ab92821)
Predicted band size: 58 kDa
![Western blot - Anti-FTO antibody [5-2H10] (ab92821), expandable thumbnail](https://content.abcam.com/products/images/fto-antibody-5-2h10-ab92821--western-blot-img4788.jpg "Western blot - Anti-FTO antibody [5-2H10] (ab92821)")
Western blot - Anti-FTO antibody [5-2H10] (ab92821)
All lanes: Western blot - Anti-FTO antibody [5-2H10] (ab92821) at 1/1000 dilution
All lanes: Rat testes lysate
Predicted band size: 58 kDa
Observed band size: 58 kDa
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