AB185996

Anti-FTSJ3 antibody

Name: Anti-FTSJ3 antibody (ab185996) | Abcam
Brand: Abcam Limited
SKU: AB185996
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal FTSJ3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FTSJ3 aa 600-700.

View Alternative Names

SB92, FTSJ3, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, Protein ftsJ homolog 3, Putative rRNA methyltransferase 3

2 Images

Supplier Data

Immunoprecipitation - Anti-FTSJ3 antibody (AB185996)

Immunoprecipitation. ab185996 at 1 μg/ml staining FTSJ3 in 293T whole cell lysate immunoprecipitated using ab185996 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : controle IgG.

All lanes:

Immunoprecipitation - Anti-FTSJ3 antibody (ab185996)

Predicted band size: 97 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-FTSJ3 antibody (AB185996)

All lanes:

Western blot - Anti-FTSJ3 antibody (ab185996) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 97 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FTSJ3 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q8IY81

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FTSJ3 also known as mitochondrial ribosomal RNA methyltransferase 3 is an enzyme with a mass of approximately 84 kDa. The protein is part of the FtsJ/hEN1 family and plays a role in RNA modification by adding methyl groups to ribosomal RNA (rRNA) impacting ribosome assembly and function. FTSJ3 is ubiquitously expressed with high levels in tissues like the heart liver and skeletal muscle indicating its importance for metabolic activity and protein synthesis.

Biological function summary

FTSJ3 functions as an rRNA methyltransferase that is important for the maintenance of mitochondrial ribosome stability. It is a component of the mitoribosome complex which is responsible for mitochondrial protein synthesis. This activity is essential for cellular energy production as it directly affects the translation of mitochondrial-encoded genes required for oxidative phosphorylation.

Pathways

FTSJ3 plays a role in mitochondrial gene expression pathways and its activity affects the oxidative phosphorylation pathway a fundamental process for ATP production. This protein works alongside related proteins like METTL12 which has complementary roles in RNA modification ensuring the stability and efficiency of mitochondrial translation machinery.

Disruptions in FTSJ3 function associate with mitochondrial diseases that involve energy deficiencies such as Leigh syndrome and mitochondrial encephalomyopathy. The protein's dysfunction can also impact neurological disorders due to its critical role in energy production connecting it to proteins like GFM1 and MRPS7 which are also involved in mitochondrial translation and whose dysfunctions similarly contribute to mitochondrial pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.. (Microbial infection) In case of infection by HIV-1 virus, recruited to HIV-1 RNA and catalyzes 2'-O-methylation of the viral genome, allowing HIV-1 virus to escape the innate immune system (PubMed : 30626973). RNA 2'-O-methylation provides a molecular signature for discrimination of self from non-self and is used by HIV-1 to evade innate immune recognition by IFIH1/MDA5 (PubMed : 30626973). Mediates methylation of internal residues of HIV-1 RNA, with a strong preference for adenosine (PubMed : 30626973). Recruited to HIV-1 RNA via interaction with TARBP2/TRBP (PubMed : 30626973).

See full target information FTSJ3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37698466

2023

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Liyang Wan,Han Xiao,Linfeng Wang,Jianzhong Hu,Hongbin Lu

Cells 8: PubMed31590334

2019

Defective Excitatory/Inhibitory Synaptic Balance and Increased Neuron Apoptosis in a Zebrafish Model of Dravet Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Brenet,Rahma Hassan-Abdi,Julie Somkhit,Constantin Yanicostas,Nadia Soussi-Yanicostas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FTSJ3 antibody

Immunoprecipitation - Anti-FTSJ3 antibody (AB185996)

Western blot - Anti-FTSJ3 antibody (AB185996)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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