JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB186009

Anti-FTSJ3 antibody - N-terminal

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal FTSJ3 antibody. N-terminal. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FTSJ3 aa 1-50.

View Alternative Names

SB92, FTSJ3, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, Protein ftsJ homolog 3, Putative rRNA methyltransferase 3

1 Images
Immunoprecipitation - Anti-FTSJ3 antibody - N-terminal (AB186009)
  • IP

Supplier Data

Immunoprecipitation - Anti-FTSJ3 antibody - N-terminal (AB186009)

Detection of FTSJ3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab186009 at 6 μg/mg lysate for IP (Lane 1). For WB detection an anti-FTSJ3 antibody was used at 1μg/ml. Lane 2 Control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FTSJ3 antibody - N-terminal (ab186009)

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human FTSJ3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8IY81

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab186009 was affinity purified using an epitope specific to FTSJ3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FTSJ3 also known as mitochondrial ribosomal RNA methyltransferase 3 is an enzyme with a mass of approximately 84 kDa. The protein is part of the FtsJ/hEN1 family and plays a role in RNA modification by adding methyl groups to ribosomal RNA (rRNA) impacting ribosome assembly and function. FTSJ3 is ubiquitously expressed with high levels in tissues like the heart liver and skeletal muscle indicating its importance for metabolic activity and protein synthesis.
Biological function summary

FTSJ3 functions as an rRNA methyltransferase that is important for the maintenance of mitochondrial ribosome stability. It is a component of the mitoribosome complex which is responsible for mitochondrial protein synthesis. This activity is essential for cellular energy production as it directly affects the translation of mitochondrial-encoded genes required for oxidative phosphorylation.

Pathways

FTSJ3 plays a role in mitochondrial gene expression pathways and its activity affects the oxidative phosphorylation pathway a fundamental process for ATP production. This protein works alongside related proteins like METTL12 which has complementary roles in RNA modification ensuring the stability and efficiency of mitochondrial translation machinery.

Disruptions in FTSJ3 function associate with mitochondrial diseases that involve energy deficiencies such as Leigh syndrome and mitochondrial encephalomyopathy. The protein's dysfunction can also impact neurological disorders due to its critical role in energy production connecting it to proteins like GFM1 and MRPS7 which are also involved in mitochondrial translation and whose dysfunctions similarly contribute to mitochondrial pathologies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.. (Microbial infection) In case of infection by HIV-1 virus, recruited to HIV-1 RNA and catalyzes 2'-O-methylation of the viral genome, allowing HIV-1 virus to escape the innate immune system (PubMed : 30626973). RNA 2'-O-methylation provides a molecular signature for discrimination of self from non-self and is used by HIV-1 to evade innate immune recognition by IFIH1/MDA5 (PubMed : 30626973). Mediates methylation of internal residues of HIV-1 RNA, with a strong preference for adenosine (PubMed : 30626973). Recruited to HIV-1 RNA via interaction with TARBP2/TRBP (PubMed : 30626973).
See full target information FTSJ3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:3411 PubMed30143613

2018

The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates.

Applications

Unspecified application

Species

Unspecified reactive species

Magnus E Jakobsson,Jędrzej M Małecki,Levon Halabelian,Benedikt S Nilges,Rita Pinto,Srikanth Kudithipudi,Stephanie Munk,Erna Davydova,Fawzi R Zuhairi,Cheryl H Arrowsmith,Albert Jeltsch,Sebastian A Leidel,Jesper V Olsen,Pål Ø Falnes
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB185996

Anti-FTSJ3 antibody

Immunoprecipitation - Anti-FTSJ3 antibody (AB185996)

  • 0
  • 0
Primary Antibodies

AB185678

Anti-FTSJ3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FTSJ3 antibody (AB185678)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB151584

Anti-EXOSC8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EXOSC8 antibody (AB151584)

  • 0
  • 0
Primary Antibodies

AB60063

Anti-UTP3 antibody [2950C2a]

Western blot - Anti-UTP3 antibody [2950C2a] (AB60063)

  • 0
  • 1
Primary Antibodies

AB60068

Anti-FTSJ2 antibody [2372C6a]

Western blot - Anti-FTSJ2 antibody [2372C6a] (AB60068)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com