AB192867

Anti-FUBP1/FBP antibody [EPR12326]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal FUBP1/FBP antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Far upstream element-binding protein 1, FBP, FUSE-binding protein 1, DNA helicase V, hDH V, FUBP1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunohistochemical analysis of paraffin embedded human endometrium tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunohistochemical analysis of paraffin embedded human ovarian adenocarcinoma tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP using ab192867 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor^®488) (ab150077) was used as the secondary antibody, at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

Supplier Data

Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Western blot analysis of pellet from HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate immunoprecipitated using ab192867 at a 1/100 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Supplier Data

Western blot - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Carrier free

Anti-FUBP1/FBP antibody [EPR12326] - BSA and Azide free
HRP

HRP Anti-FUBP1/FBP antibody [EPR12326]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12326

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The far upstream element-binding protein 1 (FUBP1) also known as FBP or FBP west is a single-stranded DNA-binding protein with a molecular mass of approximately 65 kDa. It is expressed in a range of tissues including the liver brain and hematopoietic cells. Mechanically FUBP1 regulates the transcription of genes by binding to the far upstream element (FUSE) located in proximity to transcription start sites. It plays an important role in modulating transcription factors and aids in the stability of mRNA.

Biological function summary

FUBP1 influences cellular proliferation and differentiation. It does not form part of a larger complex but interacts with different molecules to exert its function. The protein acts as an activator of transcription fostering interaction with RNA polymerase II and other factors to elevate gene expression. FUBP1's activity helps in controlling the cell cycle aiding in the switch between proliferation and cell growth arrest as needed.

Pathways

FUBP1 participates significantly in the c-Myc regulatory and p53 signaling pathways. In the context of the c-Myc pathway FUBP1 boosts the expression of c-Myc by stabilizing its mRNA a process also influenced by the U2 auxiliary factor (U2AF65). In the p53 signaling pathway FUBP1 indirectly influences p53's role in apoptosis and cellular stress responses as its regulatory balance shifts critical cellular processes depending on external and internal cues.

FUBP1's dysregulation relates to cancer and neurological disorders. Overexpression or aberrant regulation of FUBP1 links to tumorigenesis in cancers like liver cancer partially due to its interaction with c-Myc. Additionally mutations or alterations in FUBP1 are associated with oligodendrogliomas a type of brain tumor. Proteins such as p53 also link to these disorders due to FUBP1's involvement in complex signaling networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

See full target information FUBP1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:5478-5506 PubMed39468350

2024

Phosphorylation of P-stalk proteins defines the ribosomal state for interaction with auxiliary protein factors.

Applications

Unspecified application

Species

Unspecified reactive species

Kamil Filipek,Sandra Blanchet,Eliza Molestak,Monika Zaciura,Colin Chih-Chien Wu,Patrycja Horbowicz-Drożdżal,Przemysław Grela,Mateusz Zalewski,Sebastian Kmiecik,Alan González-Ibarra,Dawid Krokowski,Przemysław Latoch,Agata L Starosta,Mateusz Mołoń,Yutian Shao,Lidia Borkiewicz,Barbara Michalec-Wawiórka,Leszek Wawiórka,Konrad Kubiński,Katarzyna Socała,Piotr Wlaź,Kyle W Cunningham,Rachel Green,Marina V Rodnina,Marek Tchórzewski

The Journal of clinical investigation 134: PubMed39146021

2024

PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Yan,Xun Liu,Xuefeng Qiu,Xuebin Zhang,Jiahui Chen,Kai Xiao,Ping Wu,Chao Peng,Xiaolin Hu,Zengming Wang,Jun Qin,Liming Sun,Luonan Chen,Denglong Wu,Shengsong Huang,Lichen Yin,Zhenfei Li

Journal of medicinal chemistry 65:15391-15415 PubMed36382923

2022

Discovery of Anthranilic Acid Derivatives as Difluoromethylornithine Adjunct Agents That Inhibit Far Upstream Element Binding Protein 1 (FUBP1) Function.

Applications

Unspecified application

Species

Unspecified reactive species

Aiste Dobrovolskaite,Holly Moots,Mukund P Tantak,Kunal Shah,Jenna Thomas,Sharifa Dinara,Chelsea Massaro,Paul M Hershberger,Patrick R Maloney,Satyamaheshwar Peddibhotla,Eliot Sugarman,Sally Litherland,Juan Pablo Arnoletti,Rajiv Kumar Jha,David Levens,Otto Phanstiel

International journal of molecular medicine 47: PubMed33649780

2021

FUBP1 mediates the growth and metastasis through TGFβ/Smad signaling in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Jinlian Chen,Nvshi Zhou,Yun Lu,Jingwen Lu,Xin Xing,Hua Chen,Xingxing Zhang

Cell death & disease 11:473 PubMed32555178

2020

NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Han,Yukang Wu,Xiang Hu,Yaqi Chen,Wenwen Jia,Qizhi He,Yiding Bian,Mengfei Wang,Xudong Guo,Jiuhong Kang,Xiaoping Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FUBP1/FBP antibody [EPR12326]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Western blot - Anti-FUBP1/FBP antibody [EPR12326] (AB192867)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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