JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181025

Anti-FUBP3 antibody [EPR13174]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal FUBP3 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

FBP3, FUBP3, Far upstream element-binding protein 3, FUSE-binding protein 3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025)

Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (AB181025)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (AB181025)

Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025)

Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (AB181025)
  • IP

Supplier Data

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (AB181025)

Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)

Predicted band size: 62 kDa

false

Western blot - Anti-FUBP3 antibody [EPR13174] (AB181025)
  • WB

Supplier Data

Western blot - Anti-FUBP3 antibody [EPR13174] (AB181025)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

  • Carrier free

    Anti-FUBP3 antibody [EPR13174] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13174

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB181122

Anti-FUBP3 antibody [EPR13174-61]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174-61] (AB181122)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB248928

Anti-KHSRP antibody [EPR9865] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] - BSA and Azide free (AB248928)

  • 0
  • 0
Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

  • 5
  • 2
Secondary Antibodies

AB99697

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP)

VeriBlot

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (AB99697)

  • 5
  • 1
Primary Antibodies

AB186733

Anti-Argonaute-2 antibody [EPR10411]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FUBP3 also known as Far Upstream Element Binding Protein 3 plays a mechanical role in cellular functions through binding to DNA and RNA. This protein belongs to the family of FUSE-binding proteins that regulate gene expression at the transcriptional and translational level. It has a molecular mass of about 65 kDa. FUBP3 is highly expressed in the nucleus of various cell types including neurons and immune cells reflecting its role in cellular processes.
Biological function summary

FUBP3 acts as a regulator of gene expression by interacting with RNA and influencing RNA stability and translation. It usually functions as part of a complex with other proteins modulating transcription factors that control cell growth and differentiation. Through these interactions FUBP3 can switch genes on and off significantly affecting cellular behavior and the cellular response to environmental changes.

Pathways

The function of FUBP3 contributes to the control of the cell cycle and apoptosis pathways. Within the cell cycle it acts alongside proteins like p53 to ensure cells progress correctly through various phase transitions. In the context of apoptosis FUBP3 interacts with Bcl-2 family proteins influencing cell survival and programmed cell death. These pathways show how FUBP3 coordinates with other proteins to maintain cellular equilibrium.

Deregulation of FUBP3 has connections to cancer and neurodegenerative diseases. Its abnormal expression can lead to tumor development by disrupting normal cell cycle and apoptotic control often involving interactions with the tumor suppressor protein p53. Furthermore alterations in FUBP3 function have links to neurodegenerative disorders potentially through interactions with tau proteins which are central to diseases like Alzheimer's disease.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
See full target information FUBP3
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112595 PubMed40510133

2025

Global analysis of excitotoxicity-induced alterations in RNA structure and RNA-protein binding in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Alvarez-Periel,Marianne C Kramer,Cagla Akay-Espinoza,Daniel P Jackson,Julia A Tasca,Benjamin A Garcia,Brian D Gregory,Kelly L Jordan-Sciutto

Molecular therapy. Nucleic acids 36:102525 PubMed40248217

2025

FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin M R Gibaut,Chuan Li,Anqi Cheng,Ines Moranguinho,Luisa P Mori,Susana T Valente

Cellular & molecular immunology 19:883-897 PubMed35637281

2022

Lnc-EST12, which is negatively regulated by mycobacterial EST12, suppresses antimycobacterial innate immunity through its interaction with FUBP3.

Applications

Unspecified application

Species

Unspecified reactive species

Qili Yao,Yan Xie,Dandan Xu,Zilu Qu,Jian Wu,Yuanyuan Zhou,Yuying Wei,Huan Xiong,Xiao-Lian Zhang

The international journal of biochemistry & cell b 65:268-74 PubMed26117454

2015

Exposure to arginine analog canavanine induces aberrant mitochondrial translation products, mitoribosome stalling, and instability of the mitochondrial proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Konovalova,Taru Hilander,Fabricio Loayza-Puch,Koos Rooijers,Reuven Agami,Henna Tyynismaa
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com