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Rabbit Recombinant Monoclonal FUBP3 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-FUBP3 antibody [EPR13174] (AB181025), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (AB181025), expandable thumbnail
  • Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (AB181025), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (AB181025), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.

Alternative names

FBP3, FUBP3, Far upstream element-binding protein 3, FUSE-binding protein 3

Recommended products

Rabbit Recombinant Monoclonal FUBP3 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13174
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FUBP3 also known as Far Upstream Element Binding Protein 3 plays a mechanical role in cellular functions through binding to DNA and RNA. This protein belongs to the family of FUSE-binding proteins that regulate gene expression at the transcriptional and translational level. It has a molecular mass of about 65 kDa. FUBP3 is highly expressed in the nucleus of various cell types including neurons and immune cells reflecting its role in cellular processes.

Biological function summary

FUBP3 acts as a regulator of gene expression by interacting with RNA and influencing RNA stability and translation. It usually functions as part of a complex with other proteins modulating transcription factors that control cell growth and differentiation. Through these interactions FUBP3 can switch genes on and off significantly affecting cellular behavior and the cellular response to environmental changes.

Pathways

The function of FUBP3 contributes to the control of the cell cycle and apoptosis pathways. Within the cell cycle it acts alongside proteins like p53 to ensure cells progress correctly through various phase transitions. In the context of apoptosis FUBP3 interacts with Bcl-2 family proteins influencing cell survival and programmed cell death. These pathways show how FUBP3 coordinates with other proteins to maintain cellular equilibrium.

Associated diseases and disorders

Deregulation of FUBP3 has connections to cancer and neurodegenerative diseases. Its abnormal expression can lead to tumor development by disrupting normal cell cycle and apoptotic control often involving interactions with the tumor suppressor protein p53. Furthermore alterations in FUBP3 function have links to neurodegenerative disorders potentially through interactions with tau proteins which are central to diseases like Alzheimer's disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025), expandable thumbnail

    Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Blocking/ Dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025) at 1/5000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025), expandable thumbnail

    Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Predicted band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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