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AB250292

Anti-FUBP3 antibody [EPR13174] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FUBP3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FBP3, FUBP3, Far upstream element-binding protein 3, FUSE-binding protein 3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)

This data was developed using ab181025, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)

This data was developed using ab181025, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)

This data was developed using ab181025, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)
  • IP

Supplier Data

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)

This data was developed using ab181025, the same antibody clone in a different buffer formulation.

Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (<a href='/en-us/products/primary-antibodies/fubp3-antibody-epr13174-ab181025'>ab181025</a>)

Predicted band size: 62 kDa

false

Western blot - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)
  • WB

Supplier Data

Western blot - Anti-FUBP3 antibody [EPR13174] - BSA and Azide free (AB250292)

This data was developed using ab181025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FUBP3 antibody [EPR13174] (<a href='/en-us/products/primary-antibodies/fubp3-antibody-epr13174-ab181025'>ab181025</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13174

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250292 is the carrier-free version of ab181025.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FUBP3 also known as Far Upstream Element Binding Protein 3 plays a mechanical role in cellular functions through binding to DNA and RNA. This protein belongs to the family of FUSE-binding proteins that regulate gene expression at the transcriptional and translational level. It has a molecular mass of about 65 kDa. FUBP3 is highly expressed in the nucleus of various cell types including neurons and immune cells reflecting its role in cellular processes.
Biological function summary

FUBP3 acts as a regulator of gene expression by interacting with RNA and influencing RNA stability and translation. It usually functions as part of a complex with other proteins modulating transcription factors that control cell growth and differentiation. Through these interactions FUBP3 can switch genes on and off significantly affecting cellular behavior and the cellular response to environmental changes.

Pathways

The function of FUBP3 contributes to the control of the cell cycle and apoptosis pathways. Within the cell cycle it acts alongside proteins like p53 to ensure cells progress correctly through various phase transitions. In the context of apoptosis FUBP3 interacts with Bcl-2 family proteins influencing cell survival and programmed cell death. These pathways show how FUBP3 coordinates with other proteins to maintain cellular equilibrium.

Deregulation of FUBP3 has connections to cancer and neurodegenerative diseases. Its abnormal expression can lead to tumor development by disrupting normal cell cycle and apoptotic control often involving interactions with the tumor suppressor protein p53. Furthermore alterations in FUBP3 function have links to neurodegenerative disorders potentially through interactions with tau proteins which are central to diseases like Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
See full target information FUBP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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