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AB248403

Anti-Fukutin antibody [EPR7913] - BSA and Azide free

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Rabbit Recombinant Monoclonal Fukutin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

FCMD, FKTN, Ribitol-5-phosphate transferase FKTN, Fukutin, Fukuyama-type congenital muscular dystrophy protein, Ribitol-5-phosphate transferase

3 Images
Western blot - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)
  • WB

Unknown

Western blot - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)

This data was developed using ab131280, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Fukutin antibody [EPR7913] (<a href='/en-us/products/primary-antibodies/fukutin-antibody-epr7913-ab131280'>ab131280</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

Fetal heart tissue lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)

This data was developed using ab131280, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Fukutin with ab131280 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

OI-RD Scanning - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Fukutin antibody [EPR7913] - BSA and Azide free (AB248403)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7913

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab248403 is the carrier-free version of ab131280.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fukutin also known as FKRP or FKTN is a glycosyltransferase enzyme weighing approximately 55 kDa. This protein participates in the process of adding sugar chains to proteins primarily glycosylating α-dystroglycan. Fukutin is highly expressed in muscle tissues including skeletal and cardiac muscles and plays a role in the central nervous system. Its activity is important for the proper function of these tissues.
Biological function summary

Fukutin plays a fundamental role in maintaining the structure and function of muscle cells by participating in the glycosylation of α-dystroglycan. This modification allows α-dystroglycan to bind to extracellular matrix proteins. Fukutin operates as part of the dystrophin-glycoprotein complex which is essential for muscle integrity and stability. This complex anchors the cytoskeleton of a muscle cell to the surrounding extracellular matrix.

Pathways

This protein fukutin participates in the dystroglycan pathway affecting the integrity of muscle cells. It works closely with other proteins like LARGE1 which also modifies α-dystroglycan. Fukutin's activity ensures that α-dystroglycan has the necessary post-translational modifications to perform its function within the laminin-binding pathway linking the extracellular matrix to the cytoskeleton.

Alterations in fukutin mutations can lead to muscular dystrophies such as Fukuyama congenital muscular dystrophy and Walker-Warburg syndrome. In these conditions the fukutin-related glycosylation of α-dystroglycan is disrupted which impairs muscle cell stability and function. Other proteins like dystrophin and sarcoglycans associated with the dystrophin-glycoprotein complex also play roles in these disorders further linking fukutin to muscle disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (PubMed : 17034757, PubMed : 25279699, PubMed : 26923585, PubMed : 27194101, PubMed : 29477842). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed : 17034757). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed : 25279699). Could be involved in brain development (Probable).
See full target information FKTN
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Product promise

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