Rabbit Polyclonal FUNDC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUNDC1 aa 1-50.
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- Open medicine (Warsaw, Poland) 18:202307982023Silencing USP19 alleviates cigarette smoke extract-induced mitochondrial dysfunction in BEAS-2B cells by targeting FUNDC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanjing You,Huijuan Wang,Qing Wang,Zongyang Yu,Zhongquan Zhao,Liying Zhuang,Shengyuan Zeng,Jinyang Zheng,Wen WenPubMed 37808166
- Journal of biochemical and molecular toxicology 37:e235032023Poricoic acid A induces mitophagy to ameliorate podocyte injury in diabetic kidney disease via downregulating FUNDC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuwen Wu,Haohua Deng,Jiazhong Sun,Jun Tang,Xin Li,Yancheng XuPubMed 37706594
- Cellular and molecular biology (Noisy-le-Grand, France) 69:237-2452023Jionoside A1 alleviates ischemic stroke ischemia/reperfusion injury by promoting Nix-mediated mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyuan Yu,Xiaojun Liu,Xiujuan Mi,Xiaoqiong Luo,Zhiyun Lian,Jun Tang,Guixue WangPubMed 37715375
- iScience 26:1077722023Focused low-intensity pulsed ultrasound alleviates osteoarthritis via restoring impaired FUNDC1-mediated mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixia Ye,Dongqian Li,Xia Wei,Lehua Yu,Lang JiaPubMed 37720103
- Redox biology 62:1026932023Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Ma,Kaifeng Li,Wenxiang Wei,Jiaheng Zhou,Ze Li,Tuo Zhang,Yunshu Wangsun,Fei Tian,Qianqian Dong,Haifeng Zhang,Wenjuan XingPubMed 37030149
- Allergologia et immunopathologia 51:177-1862023NLRX1 increases human retinal pigment epithelial autophagy and reduces HO-induced oxidative stress and inflammation by suppressing FUNDC1 phosphorylation and NLRP3 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Wang,Fengying He,Liping WuPubMed 36617838
- Molecular metabolism 64:1015532022Empagliflozin activates Wnt/β-catenin to stimulate FUNDC1-dependent mitochondrial quality surveillance against type-3 cardiorenal syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Cai,Feng Wu,Bingjie Zhuang,Qing Ou,Xiaojie Peng,Nengxian Shi,Lan Peng,Ziying Li,Jin Wang,Shumin Cai,Ying TanPubMed 35863636
- Oxidative medicine and cellular longevity 2022:81925522022Hypoxia Acclimation Protects against Heart Failure Postacute Myocardial Infarction via Fundc1-Mediated Mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Li,Yinghai Liu,Qingqing Huang,Xiaobo Yi,Fuen Qin,Zuling Zhong,Lu Lin,Haihong Yang,Gu Gong,Wei WuPubMed 35422895
- International journal of molecular medicine 49:2022Hypoxia‑induced mitophagy regulates proliferation, migration and odontoblastic differentiation of human dental pulp cells through FUN14 domain‑containing 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwen Liu,Liuchi Chen,Qimei Gong,Hongwei Jiang,Yihua HuangPubMed 35362539
- Redox biology 52:1022882022Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Cai,Zhongzhou Guo,Xing Chang,Ziying Li,Feng Wu,Jing He,Tiantian Cao,Kangrong Wang,Nengxian Shi,Hao Zhou,Sam Toan,David Muid,Ying TanPubMed 35325804
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FUNDC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVP5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.
Alternative names
FUN14 domain-containing protein 1, FUNDC1
Recommended products
Rabbit Polyclonal FUNDC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUNDC1 aa 1-50.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FUNDC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVP5
- Purification technique
Affinity purification Protein G
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FUNDC1 also known as FUN14 domain-containing protein 1 is a protein involved in mitochondrial dynamics. It weighs approximately 15 kDa. FUNDC1 is primarily expressed in the mitochondrial outer membrane and shows high expression levels in tissues with high energy demands such as the heart and skeletal muscles. Its function is linked to the regulation of mitophagy a process that removes damaged mitochondria.
- Biological function summary
FUNDC1 plays a role in the selective elimination of mitochondria through mitophagy. It acts as a receptor for damaged mitochondria binding to LC3 and enabling the autophagic machinery. FUNDC1 is not known to be part of a larger protein complex but it interacts with other mitophagy-related proteins such as PGAM5 and ULK1 to facilitate mitochondrial clearance.
- Pathways
FUNDC1 participates in the mitophagy pathway and the broader mitochondrial quality control pathway. In the mitophagy pathway its interaction with proteins like LC3 facilitates the engulfment of dysfunctional mitochondria by autophagosomes. The relationship of FUNDC1 with ULK1 highlights its role in the initiation of the mitophagy signaling cascade ensuring mitochondrial integrity and cellular homeostasis.
- Associated diseases and disorders
FUNDC1 has associations with neurodegenerative diseases and cardiovascular disorders. Defects in the mitophagy process where FUNDC1 plays a significant role can contribute to the development of Parkinson's disease. Furthermore its connection to cardiovascular diseases involves interactions with BCL2L1 a protein that regulates apoptosis in heart tissue under stress conditions. Dysfunctional FUNDC1 activity may lead to the accumulation of damaged mitochondria contributing to cellular dysfunction observed in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab224722)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling FUNDC1 with ab224722 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
Western blot - Anti-FUNDC1 antibody (ab224722)
All lanes: Western blot - Anti-FUNDC1 antibody (ab224722) at 1/1000 dilution
Lane 1: Mouse Brain tissue lysate
Lane 2: Mouse Liver tissue lysate
Lane 3: Rat Brain tissue lysate
Lane 4: Rat Liver tissue lysate
SecondaryAll lanes: Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 18 kDa
Western blot - Anti-FUNDC1 antibody (ab224722)
All lanes: Western blot - Anti-FUNDC1 antibody (ab224722) at 1/1000 dilution
Lane 1: HeLa whole cell lysate
Lane 2: MCF7 whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: U251 whole cell lysate
Lane 5: K562 whole cell lysate
SecondaryAll lanes: Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 18 kDa
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