Anti-FUNDC1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human kidney tissue labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human small intestine tissue labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human colorectal cancer labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• WB

Supplier Data

Western blot - Anti-FUNDC1 antibody (AB224722)

All lanes:

Western blot - Anti-FUNDC1 antibody (ab224722) at 1/1000 dilution

Lane 1:

Mouse Brain tissue lysate at 30 µg

Lane 2:

Mouse Liver tissue lysate at 30 µg

Lane 3:

Rat Brain tissue lysate at 30 µg

Lane 4:

Rat Liver tissue lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/40000 dilution

Observed band size: 17 kDa,17 g/mol

false

Exposure time: 60s