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Rabbit Polyclonal FURIN antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human FURIN aa 750 to C-terminus.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human FURIN aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09958
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

2 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Representing Furin in BSC-40 cells transfected with the human Furin gene.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed:20489134, PubMed:21763278).(Microbial infection) Cleaves and activates diphtheria toxin DT.(Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA).(Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.(Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP.(Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.

Alternative names

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Rabbit Polyclonal FURIN antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human FURIN aa 750 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human FURIN aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09958
Purification technique

Affinity purification Immunogen

Specificity

Detects Furin from canine and mouse cells as well as transfected human Furin.
This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry - Anti-Furin antibody (ab3467), expandable thumbnail

    Immunocytochemistry - Anti-Furin antibody (ab3467)

    Immunocytochemical analysis of methanol-fixed 2% BSA blocked HeLa cells strining furin with ab3467 at 2 μg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and the cytoskeleton with alpha-Tubulin Monoclonal Antibody and Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate at 1/400 dilution (red). Negative control uses no primary antibody

  • Immunocytochemistry - Anti-Furin antibody (ab3467), expandable thumbnail

    Immunocytochemistry - Anti-Furin antibody (ab3467)

    Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.

  • Western blot - Anti-Furin antibody (ab3467), expandable thumbnail

    Western blot - Anti-Furin antibody (ab3467)

    All lanes: Western blot - Anti-Furin antibody (AB3467) at 1/500 dilution

    Lane 1: HeLa lysate at 30 µg

    Lane 2: HEK-293 extract at 30 µg

    Lane 3: K-562 extract at 30 µg

    Lane 4: SH-SY5Y extract at 30 µg

    Lane 5: conditioned media from HeLa cell line at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa

  • Immunocytochemistry - Anti-Furin antibody (ab3467), expandable thumbnail
    Image courtesy of an anonymous customer review.

    Immunocytochemistry - Anti-Furin antibody (ab3467)

    ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.

  • Western blot - Anti-Furin antibody (ab3467), expandable thumbnail

    Western blot - Anti-Furin antibody (ab3467)

    The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.

    All lanes: Western blot - Anti-Furin antibody (AB3467) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa

    Observed band size: 63 kDa

    Exposure time: 90s

  • Western blot - Anti-Furin antibody (ab3467), expandable thumbnail
    This image is courtesy of a customer review submitted by Lawrence Welch

    Western blot - Anti-Furin antibody (ab3467)

    4-20% tris-glycine, denaturing conditions

    All lanes: Western blot - Anti-Furin antibody (AB3467) at 1/1000 dilution

    Lane 1: HAP1 at 25000 Cells

    Lanes 2 - 3: independent furin CRISPR KO clones (Horizon) at 25000 Cells

    Secondary

    All lanes: rabbit IgG HRP Linked Whole Ab at 1/10000 dilution

    Developed using the ECL technique.

    Exposure time: 1min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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