Rabbit Recombinant Monoclonal FURIN antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FURIN.
Images
![Dot Blot - Anti-FURIN antibody [EPR28939-503] (AB324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--dot-blot-img447118.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-FURIN antibody [EPR28939-503] (AB324231)")
![Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--western-blot-img447085.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231)")
![Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--western-blot-img447084.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FURIN. Database link P09958
Reactivity data
Select an application| Dot | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed:20489134, PubMed:21763278). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity). (Microbial infection) Cleaves and activates diphtheria toxin DT. (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA). (Microbial infection) Cleaves and activates HIV-1 virus Envelope glycoprotein gp160. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells. (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F.
Alternative names
FUR, PACE, PCSK3, FURIN, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme
Rabbit Recombinant Monoclonal FURIN antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FURIN.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FURIN. Database link P09958
- Clone number
- EPR28939-503
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-FURIN antibody [EPR28939-503] (ab324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--dot-blot-img447118.jpg "Dot Blot - Anti-FURIN antibody [EPR28939-503] (ab324231)")
Dot Blot - Anti-FURIN antibody [EPR28939-503] (ab324231)
FURIN Dot Blot staining using rabbit Anti-FURIN antibody
Dot blot analysis of FURIN using ab324231 at 1:1000 (0.523 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: No-tagged human PCSK3 recombinant protein
Lane2: His-tagged human PCSK1 recombinant protein
Lane3: His-tagged human PCSK4 recombinant protein
Lane4: His-tagged human PCSK5 recombinant protein
Lane5: His-tagged human PCSK6 recombinant proteinExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human PCSK1, PCSK4, PCSK5, and PCSK6.
All lanes: Dot Blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution
Lane 1: No-tagged human PCSK3 recombinant protein
Lane 2: His-tagged human PCSK1 recombinant protein
Lane 3: His-tagged human PCSK4 recombinant protein
Lane 4: His-tagged human PCSK5 recombinant protein
Lane 5: His-tagged human PCSK6 recombinant protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
![Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--western-blot-img447085.jpg "Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231)")
Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231)
FURIN Western blot staining using rabbit Anti-FURIN antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: LoVo (PMID: 20585585).
Low expression: IMR-32 and Jurkat.
This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution
Lane 1: KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg
Lane 2: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 3: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 85 kDa, 36 kDa
Exposure time: 37s
![Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-epr28939-503-ab324231--western-blot-img447084.jpg "Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231)")
Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231)
FURIN Western blot staining using rabbit Anti-FURIN antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Daudi, Ramos (PMID: 7931173).
Low expression: Human lung.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 4: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 5: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 6: Human placenta tissue lysate at 20 µg
Lane 7: Human lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 85 kDa, 36 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
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