Rabbit Recombinant Multiclonal FURIN antibody. Suitable for Dot, ICC/IF, Flow Cyt (Intra), WB and reacts with Recombinant fragment - Human, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-FURIN antibody [RM2083] (AB324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--flow-cytometry-intracellular-img447053.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FURIN antibody [RM2083] (AB324440)")
![Immunocytochemistry/ Immunofluorescence - Anti-FURIN antibody [RM2083] (AB324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--immunocytochemistry-immunofluorescence-img447047.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FURIN antibody [RM2083] (AB324440)")
![Western blot - Anti-FURIN antibody [RM2083] (AB324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--western-blot-img447043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FURIN antibody [RM2083] (AB324440)")
![Western blot - Anti-FURIN antibody [RM2083] (AB324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--western-blot-img447044.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FURIN antibody [RM2083] (AB324440)")
![Dot Blot - Anti-FURIN antibody [RM2083] (AB324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--dot-blot-img447056.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-FURIN antibody [RM2083] (AB324440)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed:20489134, PubMed:21763278). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity). (Microbial infection) Cleaves and activates diphtheria toxin DT. (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA). (Microbial infection) Cleaves and activates HIV-1 virus Envelope glycoprotein gp160. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells. (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F.
Alternative names
FUR, PACE, PCSK3, FURIN, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme
Rabbit Recombinant Multiclonal FURIN antibody. Suitable for Dot, ICC/IF, Flow Cyt (Intra), WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- RM2083
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-FURIN antibody [RM2083] (ab324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--flow-cytometry-intracellular-img447053.jpg "Flow Cytometry (Intracellular) - Anti-FURIN antibody [RM2083] (ab324440)")
Flow Cytometry (Intracellular) - Anti-FURIN antibody [RM2083] (ab324440)
FURIN Flow Cytometry (Intracellular) staining using rabbit Anti-FURIN antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) (Right) / Ramos (human Burkitt's lymphoma B lymphocyte) (Left) cells labelling FURIN with ab324440 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: Ramos.
![Immunocytochemistry/ Immunofluorescence - Anti-FURIN antibody [RM2083] (ab324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--immunocytochemistry-immunofluorescence-img447047.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FURIN antibody [RM2083] (ab324440)")
Immunocytochemistry/ Immunofluorescence - Anti-FURIN antibody [RM2083] (ab324440)
FURIN Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-FURIN antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling FURIN with ab324440 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in K-562 and no staining in Ramos cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: Ramos.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Western blot - Anti-FURIN antibody [RM2083] (ab324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--western-blot-img447043.jpg "Western blot - Anti-FURIN antibody [RM2083] (ab324440)")
Western blot - Anti-FURIN antibody [RM2083] (ab324440)
FURIN Western blot staining using rabbit Anti-FURIN antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Ramos (PMID: 7931173).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1, 2: 103 seconds Lane 3-6: 37 seconds
All lanes: Western blot - Anti-FURIN antibody [RM2083] (ab324440) at 1/1000 dilution
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lydate at 40 µg
Lane 2: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lydate at 40 µg
Lane 3: KARPAS-299 (human T cell lymphoma cell) whole cell lydate at 40 µg
Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lydate at 40 µg
Lane 5: Untreated KARPAS-299 whole cell lydate at 40 µg
Lane 6: KARPAS-299 treated with Protein Deglycosylation MIX II at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85 kDa, 36 kDa
![Western blot - Anti-FURIN antibody [RM2083] (ab324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--western-blot-img447044.jpg "Western blot - Anti-FURIN antibody [RM2083] (ab324440)")
Western blot - Anti-FURIN antibody [RM2083] (ab324440)
FURIN Western blot staining using rabbit Anti-FURIN antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Human lung.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FURIN antibody [RM2083] (ab324440) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human lung tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85 kDa, 36 kDa
Exposure time: 26s
![Dot Blot - Anti-FURIN antibody [RM2083] (ab324440), expandable thumbnail](https://content.abcam.com/products/images/furin-antibody-rm2083-ab324440--dot-blot-img447056.jpg "Dot Blot - Anti-FURIN antibody [RM2083] (ab324440)")
Dot Blot - Anti-FURIN antibody [RM2083] (ab324440)
FURIN Dot Blot staining using rabbit Anti-FURIN antibody
Dot blot analysis of FURIN using ab324440 at 1:1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: No-tagged human PCSK3 recombinant protein
Lane2: His-tagged human PCSK1 recombinant protein
Lane3: His-tagged human PCSK4 recombinant protein
Lane4: His-tagged human PCSK5 recombinant protein
Lane5: His-tagged human PCSK6 recombinant proteinExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human PCSK1, PCSK4, PCSK5, and PCSK6.
In Dot blot, anti-6X His tag® antibody [AD1.1.10] (Anti-6X His tag® antibody [AD1.1.10] ab15149) staining at 1/1000 dilution.
All lanes: Dot Blot - Anti-FURIN antibody [RM2083] (ab324440) at 1/1000 dilution
Lane 1: No-tagged human PCSK3 recombinant protein with NFDM/TBST
Lane 2: His-tagged human PCSK1 recombinant protein with NFDM/TBST
Lane 3: His-tagged human PCSK4 recombinant protein with NFDM/TBST
Lane 4: His-tagged human PCSK5 recombinant protein with NFDM/TBST
Lane 5: His-tagged human PCSK6 recombinant protein with NFDM/TBST
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com