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AB181461

Anti-FUT4 antibody [6B11B4]

4

(1 Review)

|

(3 Publications)

Mouse Monoclonal FUT4 antibody. Suitable for WB and reacts with Transfected cell line - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUT4 aa 150-350.

View Alternative Names

ELFT, FCT3A, FUT4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, Fuc-TIV, FucT-IV

3 Images
Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)
  • WB

Supplier Data

Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)

All lanes:

Western blot - Anti-FUT4 antibody [6B11B4] (ab181461) at 1/500 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 59 kDa

false

Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)
  • WB

Supplier Data

Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)

Expected MWt is 37 kDa.

All lanes:

Western blot - Anti-FUT4 antibody [6B11B4] (ab181461) at 1/500 dilution

All lanes:

FUT4 recombinant protein

Predicted band size: 59 kDa

false

Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)
  • WB

Supplier Data

Western blot - Anti-FUT4 antibody [6B11B4] (AB181461)

All lanes:

Western blot - Anti-FUT4 antibody [6B11B4] (ab181461) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

FUT4 (Amino acids: 199-302)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6B11B4

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FUT4 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P22083

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR170435-13 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FUT4 protein also known as fucosyltransferase 4 or CD15 plays an essential role in glycosylation processes by adding fucose to glycolipids and glycoproteins. This enzyme is responsible for the synthesis of Lewis X antigens. FUT4 has a molecular mass of around 44 kDa and is expressed prominently in myeloid cells such as neutrophils and monocytes. It exhibits a significant presence in the bone marrow and expressed on the surface of certain leukocytes.
Biological function summary

The activity of FUT4 is important for cell adhesion migration and immune response modulation. It helps in the formation of selectin ligands which mediate cell-to-cell interactions through the binding of selectins on endothelial cells. FUT4 does not form a larger protein complex but interacts with substrates and cofactors to achieve its function. Its role is particularly significant in the context of inflammation and hematopoiesis.

Pathways

FUT4 is involved in the biosynthesis of glycoconjugates especially influencing the E-selectin and P-selectin pathways. These pathways are integral to leukocyte trafficking during inflammatory responses. FUT4 activity shows a functional relationship with other fucosyltransferases including FUT3 and FUT5 which also participate in modifying glycolipids and glycoproteins. This makes FUT4 a part of the larger network regulating cell-surface carbohydrate structures.

FUT4 has been linked to chronic inflammatory diseases and Leukocyte Adhesion Deficiency II. Aberrations in FUT4 expression can lead to altered selectin-mediated adhesion impacting immune cell recruitment. Additionally FUT4 involvement in cancer has attracted attention as changes in its activity can impact tumor cell metastasis. It is also worthwhile to note pathways with E-selectin can influence its disease association highlighting possible therapeutic targets in E-selectin-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform Short. Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed : 1702034, PubMed : 1716630, PubMed : 29593094). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed : 1716630, PubMed : 29593094). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed : 11278338, PubMed : 1716630).. Isoform Long. Does not generate Lewis X antigens.
See full target information FUT4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 16:384-394 PubMed33981845

2021

Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Han,Lijuan Liu

Experimental and therapeutic medicine 21:407 PubMed33692838

2021

MicroRNA-200b relieves LPS-induced inflammatory injury by targeting FUT4 in knee articular chondrocytes .

Applications

Unspecified application

Species

Unspecified reactive species

Yintai Li,Suizhuan Wei,Zhongping Zhang

Oncotarget 8:28247-28259 PubMed28423676

2017

Tumor-associated macrophages promote Ezrin phosphorylation-mediated epithelial-mesenchymal transition in lung adenocarcinoma through FUT4/LeY up-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Aman Wang,Chang Lu,Zhen Ning,Wei Gao,Yunpeng Xie,Ningning Zhang,Jinxiao Liang,Faisal S Abbasi,Qiu Yan,Jiwei Liu
View all publications
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